1. Berberine improves intestinal epithelial tight junctions by upregulating A20 expression in IBS-D mice
- Author
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Qiuke Hou, Shuilian Zhu, Changrong Zhang, Yongquan Huang, Yajuan Guo, Peiwu Li, Xinlin Chen, Yi Wen, Quanbin Han, and Fengbin Liu
- Subjects
Berberine ,IBS-D ,A20 ,TNF-α-NF-κB-MLCK pathway ,Intestinal epithelial barrier ,Therapeutics. Pharmacology ,RM1-950 - Abstract
To investigate effects of berberine exerts on A20 expression and regulation of intestinal epithelial tight junctions via the TNF-α-NF-κB-MLCK pathway in Diarrhea-Predominant Irritable Bowel Syndrome (IBS-D).C57BL/6 wild type (WT) and A20 IEC-KO mice (48 each) were randomly divided into normal control (NC), model control (MC), rifaximin and berberine groups (12 mice per group). An experimental model of IBS-D was established using 4% acetic acid and evaluated by haematoxylin-eosin (HE) staining. rifaximin and berberine mice were treated with rifaximin and berberine, respectively.Intestinal epithelial space of WT berberine mice improved more than A20 IEC-KO berberine mice compared to MC mice. WT berberine mice exhibited greater expression of A20 compared with MC mice(P
- Published
- 2019
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