9 results on '"Matthias Hamburger"'
Search Results
2. Alkaloids in commercial preparations of California poppy – Quantification, intestinal permeability and microbiota interactions
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Antoine Chauveau, Annelies Geirnaert, Angela Babst, Andrea Treyer, Christophe Lacroix, Matthias Hamburger, and Olivier Potterat
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Californidine ,Escholtzine ,Protopine ,Eschscholzia californica ,Caco-2 cells ,Gut microbiota ,Therapeutics. Pharmacology ,RM1-950 - Abstract
California poppy products are commonly used for the treatment of nervousness, anxiety and sleeping disorders. Pharmacologically relevant constituents include the main alkaloids californidine, escholtzine and protopine. However, only limited information is available about the alkaloid content in commercial preparations and their intestinal absorption. Moreover, a possible metabolization of these alkaloids by the gut microbiota, and their impact on microbial activity and viability have not been investigated. Californidine, escholtzine and protopine were quantified by UHPLC-MS/MS in eight commercial California poppy products. The intestinal permeability of alkaloids was studied in Caco-2 cell as a model for absorption in the small intestine. The gut microbial biotransformation was explored in artificial gut microbiota from the in vitro PolyFermS model. In addition, the impact of these alkaloids and a California poppy extract on the microbial production of short-chain fatty acids (SCFAs) and the viability of microbiota was investigated. Contents of californidine, escholtzine and protopine in California poppy products were in the ranges of 0.13–2.55, 0.05–0.63 and 0.008–0.200 mg/g, respectively. In the Caco-2 cell model, californidine was low-to-moderately permeable while escholtzine and protopine were highly permeable. An active transport process was potentially involved in the transfer of the three alkaloids. The three compounds were not metabolized by the artificial gut microbiota over 24 h. Neither the California poppy extract nor the alkaloids markedly impacted microbial SCFA production and bacterial viability.
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- 2023
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3. Intestinal permeability and gut microbiota interactions of pharmacologically active compounds in valerian and St. John’s wort
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Antoine Chauveau, Andrea Treyer, Annelies Geirnaert, Lea Bircher, Angela Babst, Vanessa Fabienne Abegg, Ana Paula Simões-Wüst, Christophe Lacroix, Olivier Potterat, and Matthias Hamburger
- Subjects
Valerenic acid ,Hyperforin ,Hypericin ,Caco-2 cells ,Gut microbiota ,Short-chain fatty acids ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Phytomedicines such as valerian and St. John’s wort are widely used for the treatment of sleeping disorders, anxiety and mild depression. They are perceived as safe alternatives to synthetic drugs, but limited information is available on the intestinal absorption and interaction with human intestinal microbiota of pharmacologically relevant constituents valerenic acid in valerian, and hyperforin and hypericin in St. John’s wort. The intestinal permeability of these compounds and the antidepressant and anxiolytic drugs citalopram and diazepam was investigated in the Caco-2 cell model with bidirectional transport experiments. In addition, interaction of compounds and herbal extracts with intestinal microbiota was evaluated in artificial human gut microbiota. Microbiota-mediated metabolisation of compounds was assessed, and bacterial viability and short-chain fatty acids (SCFA) production were measured in the presence of compounds or herbal extracts. Valerenic acid and hyperforin were highly permeable in Caco-2 cell monolayers. Hypericin showed low-to-moderate permeability. An active transport process was potentially involved in the transfer of valerenic acid. Hyperforin and hypericin were mainly transported through passive transcellular diffusion. All compounds were not metabolized over 24 h in the artificial gut microbiota. Microbial SCFA production and bacterial viability was not substantially impaired nor promoted by exposure to the compounds or herbal extracts.
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- 2023
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4. A neolignan from Connarus tuberosus as an allosteric GABAA receptor modulator at the neurosteroid binding site
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Teresa Faleschini, Nova Syafni, Heidi Luise Schulte, Aleksandra Garifulina, Steffen Hering, Laila Salmen Espindola, and Matthias Hamburger
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Connarus tuberosus ,Connaraceae ,Brazilian Cerrado ,Neoflavonoid ,GABAA receptor ,Therapeutics. Pharmacology ,RM1-950 - Abstract
In a screening of a small library of extracts from plants of the Amazonian and Cerrado biomes, a hexane extract of Connarus tuberosus roots was found to significantly potentiate the GABA induced fluorescence in a fluorescence (FLIPR) assay in CHO cells stably expressing the α1β2γ2 subtype of human GABAA receptors. With the aid of HPLC-based activity profiling the activity was linked to the neolignan connarin. In CHO cells the activity of connarin was not abolished by increasing concentrations of flumazenil, while the effect of diazepam was increased by increasing concentrations of connarin. The effect of connarin was abolished by pregnenolone sulfate (PREGS) in a concentration-dependent manner, and the effect of allopregnanolone was further increased by increasing concentrations of connarin. In a two-microelectrode voltage clamp assay with Xenopus laevis oocytes transiently expressing GABAA receptors composed of human α1β2γ2S and α1β2 subunits connarin potentiated the GABA-induced currents, with EC50 values of 1.2 ± 0.3 μM (α1β2γ2S) and 1.3 ± 0.4 μM (α1β2), and with a maximum enhancement of currents Emax of 1959 ± 70% (α1β2γ2S) and 185 ± 48% (α1β2). The activation induced by connarin was abolished by increasing concentrations of PREGS.
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- 2023
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5. Aryltetralin lignans from Hyptis brachiata inhibiting T lymphocyte proliferation
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Morris Keller, Moritz Winker, Amy Marisa Zimmermann-Klemd, Nino Sperisen, Mahabir P. Gupta, Pablo N. Solis, Matthias Hamburger, Olivier Potterat, and Carsten Gründemann
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T lymphocyte proliferation ,Autoimmune diseases ,Flow cytometry ,Hyptis brachiata ,Aryltetralin lignans ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Increased activation and proliferation of T lymphocytes plays an essential role in the development of chronic inflammation and autoimmune diseases. Currently used immunosuppressive drugs often do not provide long-lasting relief of symptoms and show a gradual loss of efficacy over time, and are accompanied by various side effects. Therefore, novel immunosuppressive lead substances are needed. For this purpose, an in-house library consisting of 600 extracts of plants from Panama was screened for inhibition of human T lymphocyte proliferation. As one of the hits, an ethyl acetate extract from the aerial parts of Hyptis brachiata (Lamiaceae) exhibited strong inhibitory effects. Subsequent investigation resulted in the isolation of seven aryltetralin lignans, five arylnaphthalene lignans, two flavonoids, three triterpenes, and cinnamyl cinnamate. Aryltetralin lignans inhibited T lymphocyte proliferation in a concentration-dependent manner without induction of apoptosis. No relevant inhibition was observed for the arylnaphthalene lignans, flavonoids, and triterpenes. Additional cell cycle arrest investigations revealed that isolated aryltetralin lignans potently inhibited cell division in G2/M phase similarly to podophyllotoxin. Multifluorescence panel analyses of the extract also showed weak suppressive effects on the production of IL-2 and TNF-α. Therefore, preparations made out of H. brachiata could be further explored as an interesting herbal alternative in the treatment of autoimmune diseases.
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- 2023
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6. Flavonoids from Ericameria nauseosa inhibiting PI3K/AKT pathway in human melanoma cells
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Tanja Hell, Maciej Dobrzyński, Fabian Gröflin, Jakob K. Reinhardt, Lara Dürr, Olivier Pertz, Matthias Hamburger, and Eliane Garo
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Ericameria nauseosa ,Melanoma ,PI3K/AKT ,MAPK/ERK ,HPLC-based activity profiling ,Flavonoids ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The PI3K/AKT and MAPK/ERK pathways are frequently mutated in metastatic melanoma. In a screen of over 2500 plant extracts, the dichloromethane extract of Ericameria nauseosa significantly inhibited oncogenic activity of AKT in MM121224 human melanoma cells. This extract was analyzed by analytical HPLC, and the column effluent was fractionated and tested for activity to generate the so-called HPLC-based activity profile. Compounds eluting within active time-windows of the chromatogram were subsequently isolated in a larger scale to afford 11 flavones (1-11), four flavanones (12-15), two diterpenes (16, 17), and a seco-caryophyllene (18). All isolated compounds were tested for activity, whereby only flavonoids were found active. Of these, flavones were shown to be more active than the flavanones. The most potent flavone was compound 9, that was displaying an IC50 of 14.7 ± 1.4 µM on AKT activity in MM121224 cells. The terpenoids (16-18) were found to be inactive in the assay. Both diterpenes, a grindelic acid derivative (16) and an ent-neo-clerodane (17) were identified as new natural products. Their absolute configuration was established by ECD. Compound 17 is the first description of a clerodane type diterpene in the genus Ericameria.
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- 2022
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7. Pheophorbide a identified in an Eupatorium perfoliatum extract is a novel lymphatic vascular activator
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Jihye Kim, Epameinondas Gousopoulos, Teresa M. Faleschini, Matthias Hamburger, Olivier Potterat, and Michael Detmar
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Eupatorium perfoliatum ,Plant extract ,Pheophorbide a ,Lymphangiogenesis ,Lymphatic activator ,Therapeutics. Pharmacology ,RM1-950 - Abstract
The lymphatic vascular system is crucial for maintaining tissue fluid homeostasis and immune surveillance. Promoting lymphatic function represents a new strategy to treat several diseases including lymphedema, chronic inflammation and impaired wound healing. By screening a plant extract library, a petroleum ether extract from the aerial parts of Eupatorium perfoliatum (E. perfoliatum) was found to possess lymphangiogenic properties. With the aid of HPLC activity profiling the active compound was identified as pheophorbide a. Both plant extract and pheophorbide a induced the sprouting and tube formation of human primary lymphatic endothelial cells (LECs). The proliferation of the LECs was increased upon treatment with pheophorbide a but not the E. perfoliatum extract. Treatment with the MEK1/2 inhibitor U0126 reduced the LEC sprouting activity, indicating a potential mechanism of action. These studies suggest that pheophorbide a could represent novel natural therapeutic agent to treat human lymphatic vascular insufficiencies.
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- 2022
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8. Immunosuppressant flavonoids from Scutellaria baicalensis
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Nova Syafni, Seema Devi, Amy M. Zimmermann-Klemd, Jakob K. Reinhardt, Ombeline Danton, Carsten Gründemann, and Matthias Hamburger
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Scutellaria baicalensis ,T-lymphocyte proliferation ,Immunosuppressant activity ,HPLC based activity profiling ,Flavonoids ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Some plants used in Traditional Chinese Medicine serve as treatment for disease states where a suppression of the cellular immune response is desired. However, the compounds responsible for the immunosuppressant effects of these plants are not necessarily known.The immunosuppressant compounds in the roots of Scutellaria baicalensis, one of the most promising plants identified in a previous screening, were tracked by HPLC activity profiling and concomitant on-line spectroscopic analysis. Compounds were then isolated by preparative chromatography, and structures elucidated by spectroscopic methods. Twelve flavonoids (5–16) were identified from the active time windows, and structurally related flavones 2, 4, and 17, and flavanones 1 and 3 were isolated from adjacent fractions. All flavonoids possessed an unusual substitution pattern on the B-ring, with an absence of substituents at C-3 and C-4. Compounds 11, 13, 14, and 16 inhibited T-cell proliferation (IC50 values at 12.1–39 μM) at non-cytotoxic concentrations. The findings may support the use of S. baicalensis in disorders where a modulation of the cellular immune response is desirable.
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- 2021
- Full Text
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9. Immunosuppressant flavonoids from Scutellaria baicalensis
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AM Klemd, N Syafni, Jakob K. Reinhardt, Matthias Hamburger, Ombeline Danton, and Carsten Gründemann
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T-Lymphocytes ,T-lymphocyte proliferation ,RM1-950 ,Immunosuppressant activity ,Lymphocyte Activation ,Plant Roots ,High-performance liquid chromatography ,Flavones ,Structure-Activity Relationship ,Immune system ,Activity profiling ,Ic50 values ,Humans ,Cells, Cultured ,Cell Proliferation ,Flavonoids ,Pharmacology ,chemistry.chemical_classification ,Molecular Structure ,Traditional medicine ,biology ,Plant Extracts ,Chemistry ,HPLC based activity profiling ,General Medicine ,biology.organism_classification ,Biochemistry ,Active time ,Scutellaria baicalensis ,Therapeutics. Pharmacology ,Immunosuppressive Agents - Abstract
Some plants used in Traditional Chinese Medicine serve as treatment for disease states where a suppression of the cellular immune response is desired. However, the compounds responsible for the immunosuppressant effects of these plants are not necessarily known. The immunosuppressant compounds in the roots of Scutellaria baicalensis, one of the most promising plants identified in a previous screening, were tracked by HPLC activity profiling and concomitant on-line spectroscopic analysis. Compounds were then isolated by preparative chromatography, and structures elucidated by spectroscopic methods. Twelve flavonoids (5–16) were identified from the active time windows, and structurally related flavones 2, 4, and 17, and flavanones 1 and 3 were isolated from adjacent fractions. All flavonoids possessed an unusual substitution pattern on the B-ring, with an absence of substituents at C-3 and C-4. Compounds 11, 13, 14, and 16 inhibited T-cell proliferation (IC50 values at 12.1–39 μM) at non-cytotoxic concentrations. The findings may support the use of S. baicalensis in disorders where a modulation of the cellular immune response is desirable.
- Published
- 2021
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