1. Proteins from the Rhinella schneideri parotoid gland secretion exhibit anti-nociceptive effect against nociception induced by inflammation
- Author
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Jefferson Soares de Oliveira, Luís Mesquita Sousa-Filho, Anna Carolina Toledo da Cunha Pereira, Mauro Sérgio Cruz Souza Lima, Francisco E.S. Lopes, Cleverson D.T. Freitas, André Luiz dos Reis Barbosa, Gustavo Portela Ferreira, and Renan O. Silva
- Subjects
Male ,Nociception ,0301 basic medicine ,medicine.medical_specialty ,Anti-Inflammatory Agents ,Pain ,Bradykinin ,Toad ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,biology.animal ,Rhinella schneideri ,medicine ,Animals ,Edema ,Prostaglandin E2 ,Acetic Acid ,Pain Measurement ,Inflammation ,Pharmacology ,Analgesics ,biology ,Plant Extracts ,Chemistry ,Parotoid gland ,Proteins ,General Medicine ,biology.organism_classification ,Bufonidae ,Disease Models, Animal ,030104 developmental biology ,Endocrinology ,Licking ,030217 neurology & neurosurgery ,Histamine ,medicine.drug - Abstract
As proteins isolated from the Rhinella schneideri parotoid gland secretion (RsPP) exhibit anti-inflammatory activity, the goal of this work was to investigate their anti-nociceptive effects using acetic acid-induced writhing, formalin, and hot-plate tests. The intraperitoneal administration of RsPP (2.5 or 5 mg/kg) one hour prior to stimuli significantly reduced the abdominal constrictions induced by acetic acid (73.06 and 72.69% inhibition, respectively) and the inflammatory phase of paw licking time induced by formalin (69.3% inhibition, at 2.5 mg/kg). However, RsPP (1, 2.5 or 5 mg/kg) did not change the latency in response at the hot-plate test. The involvement of inflammatory mediators on the anti-nociceptive effect of RsPP was further demonstrated. RsPP (2.5 mg/kg) significantly inhibited the inflammatory peak of paw edema induced by histamine (44.0%), bradykinin (51.3%), or prostaglandin E2 (53.7%). Our data indicate that RsPP may act on the pain process by inhibiting the effect of inflammatory mediators.
- Published
- 2017
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