1. Apoptotic effect of MG-132 on human tongue squamous cell carcinoma
- Author
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Chen, Shuang-feng, Chen, Hai-ying, Liu, Xian-bin, Zhang, Ying-xin, Liu, Wei, Wang, Wei-hua, Zhang, Bin, and Wang, Le-Xin
- Subjects
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TONGUE cancer , *SQUAMOUS cell carcinoma , *CELL lines , *UBIQUITIN , *APOPTOSIS , *WESTERN immunoblotting , *POLYMERASE chain reaction - Abstract
Abstract: The aim of this study was to investigate the apoptotic effect of a proteasome inhibitor MG-132 on Tca-8113, a cell line of human tongue squamous cell carcinoma. Tca-8113 cells were treated with 10, 20, and 30μM of MG-132, or 5μM thapsigargin. Apoptosis rate was determined with annexin V/propidium iodide double staining. Expression of E3ubiquitin-protein ligase was determined by ELISA, and Grp78 and caspase-12 mRNA, and Grp78 and caspase-12 protein was assessed by RT-PCR and Western blot, respectively. Apoptosis was observed 18h after MG-132 treatment. The apoptotic rate in the 10, 20, and 30μM MG-132 group was 13.5, 19.6 and 34.7%, respectively, which was higher than in the thapsigargin (8.5%, P <0.01) or control group (0.5%, P <0.01). The expression of E3 ubiquitin-protein ligase in the 10, 20, and 30μM MG-132 group was 28.75±2.28, 18.16±0.65, 8.85±0.72, respectively, which was lower than in the thapsigargin (38.96±0.33, P <0.05 or 0.01) or control (40.88±4.52, P <0.05 or 0.01) group. The levels of Grp78 and capase-12 mRNA, Grp78 and caspase-12 protein in the MG-132 groups were higher than in the control group (P <0.01). In conclusion, MG-132 induces apoptosis in Tca-8113 cells in a concentration-dependent manner. The MG-132-induced apoptosis may involve downregulation of E3 ubiquitin ligase, and upregulation of Grp78 and caspase-12. [Copyright &y& Elsevier]
- Published
- 2011
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