1. High resolution optical projection tomography platform for multispectral imaging of the mouse gut
- Author
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Mathieu Di Franco, Jérôme Extermann, Alessio Mylonas, Yoan Neuenschwander, Enrico Pomarico, Aleksandra Radenovic, David Nguyen, Adrien Descloux, Arielle Planchette, Cédric Schmidt, and Gabriel Giardina
- Subjects
Physics ,0303 health sciences ,reconstruction ,Multispectral image ,Resolution (electron density) ,High resolution ,deconvolution ,zebrafish ,fluorescence microscopy ,01 natural sciences ,Optical projection tomography ,quantification ,Atomic and Molecular Physics, and Optics ,Article ,010309 optics ,03 medical and health sciences ,Biological specimen ,0103 physical sciences ,High spatial resolution ,Biological imaging ,light-sheet microscopy ,030304 developmental biology ,Biotechnology ,Lumen (unit) ,Biomedical engineering - Abstract
Optical projection tomography (OPT) is a powerful tool for three-dimensional imaging of mesoscopic biological samples with great use for biomedical phenotyping studies. We present a fluorescent OPT platform that enables direct visualization of biological specimens and processes at a centimeter scale with high spatial resolution, as well as fast data throughput and reconstruction. We demonstrate nearly isotropic sub-28 µm resolution over more than 60 mm3 after reconstruction of a single acquisition. Our setup is optimized for imaging the mouse gut at multiple wavelengths. Thanks to a new sample preparation protocol specifically developed for gut specimens, we can observe the spatial arrangement of the intestinal villi and the vasculature network of a 3-cm long healthy mouse gut. Besides the blood vessel network surrounding the gastrointestinal tract, we observe traces of vasculature at the villi ends close to the lumen. The combination of rapid acquisition and a large field of view with high spatial resolution in 3D mesoscopic imaging holds an invaluable potential for gastrointestinal pathology research.
- Published
- 2021