1. Simultaneous determination of lamotrigine, zonisamide, and carbamazepine in human plasma by high-performance liquid chromatography.
- Author
-
Greiner-Sosanko E, Lower DR, Virji MA, and Krasowski MD
- Subjects
- Adult, Humans, Lamotrigine, Plasma chemistry, Reproducibility of Results, Sensitivity and Specificity, Spectrophotometry, Ultraviolet methods, Zonisamide, Anticonvulsants blood, Carbamazepine blood, Chromatography, High Pressure Liquid methods, Isoxazoles blood, Triazines blood
- Abstract
A high-performance liquid chromatography assay with ultraviolet detection was developed for the simultaneous determination of the anti-epileptic drugs lamotrigine, carbamazepine and zonisamide in human plasma and serum. Lamotrigine, carbamazepine, zonisamide and the internal standard chloramphenicol were extracted from serum or plasma using liquid-liquid extraction under alkaline conditions into an organic solvent. The method was linear in the range 1-30 microg/mL for lamotrigine, 2-20 microg/mL for carbamazepine, and 1-40 microg/mL for zonisamide. Within- and between-run precision studies demonstrated coefficient of variation <10% at all tested concentrations. Other anti-epileptic medications tested did not interfere with the assay. The method is appropriate for determining lamotrigine, carbamazepine and zonisamide serum or plasma concentrations for therapeutic monitoring.
- Published
- 2007
- Full Text
- View/download PDF