1. Quantitation of glucosamine sulfate in plasma by HPLC-MS/MS after administration of powder for oral solution formulation.
- Author
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César IC, Byrro RM, de Santana E Silva Cardoso FF, Mundim IM, de Souza Teixeira L, Rezende KR, da Silva EP, Gomes SA, de Sousa VA, Bonfim RR, and Pianetti GA
- Subjects
- Administration, Oral, Female, Glucosamine administration & dosage, Glucosamine pharmacokinetics, Humans, Male, Powders administration & dosage, Reproducibility of Results, Sensitivity and Specificity, Chromatography, High Pressure Liquid methods, Glucosamine blood, Tandem Mass Spectrometry methods
- Abstract
A rapid method for the quantification of glucosamine in human plasma using high-performance liquid chromatography coupled to tandem mass spectrometry was developed and validated. The sample preparation includes a simple deproteinization step, using D-[1-¹³C] glucosamine hydrochloride as an internal standard. Chromatographic separation was performed on an ACE Ciano column using isocratic elution with acetonitrile and aqueous 2 mM ammonium acetate containing 0.025% formic acid (80:20). Selected reaction monitoring was performed using the transitions m/z 180.1 → m/z 72.1 and m/z 181.0 → m/z 74.6 to quantify glucosamine and internal standard, respectively. The method was validated and proved to be linear, accurate and precise over the range 50-5000 ng/mL of glucosamine. Recovery rates higher than 90% were obtained for both glucosamine and internal standard. No matrix effect was detected in the samples. The validated method was successfully applied to a pharmacokinetic study after oral administration of a powder for oral solution formulation containing glucosamine sulfate., (Copyright © 2011 John Wiley & Sons, Ltd.)
- Published
- 2012
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