1. Recognition and identification of active components from Radix Bupleuri using human neuroblastoma SH-SY5Y cells
- Author
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Yan, Zhang, Feihu, Liu, Xiaohong, Zhang, Tanghui, Xu, Wei, Quan, Hui, Wang, Jianguo, Shi, Zunxiao, Dai, Bin, Wu, and Qiangju, Wu
- Subjects
Psychotropic Drugs ,Plants, Medicinal ,Cell Membrane ,Reproducibility of Results ,Saponins ,Gas Chromatography-Mass Spectrometry ,Neuroblastoma ,Cell Line, Tumor ,Drug Discovery ,Humans ,Medicine, Chinese Traditional ,Oleanolic Acid ,Chromatography, Liquid ,Drugs, Chinese Herbal - Abstract
The aim of the study was to screen active components of Radix Bupleuri (a traditional Chinese herb) and discover novel anti-schizophrenic candidate drugs using human neuroblastoma SH-SY5Y cells. SH-SY5Y cells were used for preparation of the stationary phase in the cell membrane chromatography model. Retention components by the SH-SY5Y/CMC model were collected and then analyzed by GC/MS under the optimized conditions in offline conditions. After investigating the suitability and reliability of the SH-SY5Y/CMC method using amisulpride and haloperidol as standard compounds, this method was applied to screening active components from the extracts of Radix Bupleuri. Retention components of SH-SY5Y/CMC model were saikosaponin A, saikosaponin B1, saikosaponin B2, saikosaponin C and saikosaponin D, which were identified by the GC/MS method. In vitro pharmacological trials-MTT, saikosaponin B1, saikosaponin B2 and saikosaponin C could protect SY5Y cells. The protective effects of saikosaponin B1 and saikosaponin C were concentration dependent. Saikosaponin A and saikosaponin D inhibited cell viability at concentrations30 µg/mL (p 0.05). Via SH-SY5Y/CMC method and SH-SY5Y MTT trial, we rapidly detected target components from Radix Bupleuri, accurately identified them and determined their different effects on SH-SY5Y cells. Saikosaponin B1, saikosaponin B2 and saikosaponin C may be anti-schizophrenic candidate drugs.
- Published
- 2015