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A capillary zone electrophoresis method was developed for the separation and determination of strychnine and brucine in Strychnos nux‐vomica L. and its preparation. The factors that could affect the separation were studied, such as the types and concentrations of electrolytes, pH, ionic strength and organic modifier. The optimum running buffer was 20 mmol/L of ammonium acetate containing 0.2 mol/L of glacial acetic acid (pH 3.64). The applied voltage was 25 kV and the wavelength of the UV detector was set at 214 nm. The established method with dopamine hydrochloride as internal standard was linear in the range of 5–100 µg/mL for both strychnine and brucine. The recovery was 102.96% for strychnine and 98.56% for brucine. The extracts of Strychnos nux‐vomicaand its preparation could be directly injected for analysis. Copyright © 2000 John Wiley & Sons, Ltd.

A capillary zone electrophoresis method was developed for the separation and determination of strychnine and brucine in Strychnos nux-vomica L. and its preparation. The factors that could affect the separation were studied, such as the types and concentrations of electrolytes, pH, ionic strength and organic modifier. The optimum running buffer was 20 mmol/L of ammonium acetate containing 0.2 mol/L of glacial acetic acid (pH 3.64). The applied voltage was 25 kV and the wavelength of the UV detector was set at 214 nm. The established method with dopamine hydrochloride as internal standard was linear in the range of 5–100 µg/mL for both strychnine and brucine. The recovery was 102.96% for strychnine and 98.56% for brucine. The extracts of Strychnos nux-vomica and its preparation could be directly injected for analysis. Copyright © 2000 John Wiley & Sons, Ltd.

A non-aqueous capillary electrophoretic method was established for the determination of berberine in Rhizoma coptidis and its preparations. The effects of organic solvent and the concentrations of sodium acetate were studied, which showed that berberine in extracts of traditional Chinese medicine can be separated successfully in a buffer solution of 75 mmol/L of sodium acetate in methanol containing 1 mol/L of acetic acid. The simple and rapid method was linear in the range 25–200 µg/mL of berberine and had a good reproducibility, with the relative standard deviation below 2%. Non-aqueous capillary electrophoresis is a satisfactory system for the analysis of alkaloids in traditional Chinese medicine. Copyright © 1999 John Wiley & Sons, Ltd.

A capillary zone electrophoresis method was set up for the separation and determination of glycyrrhizin in Chinese medicinal preparations. Concentrations of Na2B4O7 were optimized, which showed that glycyrrhizin in the sample could be separated from interference in the running buffer of 30 mmol/L Na2B4O7. Using declofenac as internal standard, the simple method was linear in the range 25–300 µg/mL of glycyrrhizin, and good reproducibility was obtained. The extracts of Radix glycyrrhizae and its preparations could be injected directly for analysis without any pretreatment. Copyright © 1999 John Wiley & Sons, Ltd.

A non‐aqueous capillary electrophoretic method was established for the determination of berberine in Rhizoma coptidisand its preparations. The effects of organic solvent and the concentrations of sodium acetate were studied, which showed that berberine in extracts of traditional Chinese medicine can be separated successfully in a buffer solution of 75 mmol/L of sodium acetate in methanol containing 1 mol/L of acetic acid. The simple and rapid method was linear in the range 25–200 µg/mL of berberine and had a good reproducibility, with the relative standard deviation below 2%. Non‐aqueous capillary electrophoresis is a satisfactory system for the analysis of alkaloids in traditional Chinese medicine. Copyright © 1999 John Wiley & Sons, Ltd.

A capillary zone electrophoresis method was set up for the separation and determination of glycyrrhizin in Chinese medicinal preparations. Concentrations of Na2B4O7were optimized, which showed that glycyrrhizin in the sample could be separated from interference in the running buffer of 30 mmol/L Na2B4O7. Using declofenac as internal standard, the simple method was linear in the range 25–300 µg/mL of glycyrrhizin, and good reproducibility was obtained. The extracts of Radix glycyrrhizaeand its preparations could be injected directly for analysis without any pretreatment. Copyright © 1999 John Wiley & Sons, Ltd.

A micellar electrokinetic capillary chromatographic method was set up for the separation of five anthraquinone derivatives in rhubarb. Optimization of pH and sodium deoxycholate(SDC) concentrations was studied, which showed that 50 mM H3BO3‐NaOH(pH 11) containing 25 mM SDC could separate the five ingredients completely within 10 min with good reproducibility of elution time. © 1998 John Wiley & Sons, Ltd.

Ferulic acid in extracts of raw herbs was separated by capillary zone electrophoresis in the buffer solution of 10 mmol/L Na2B4O7. The simple and rapid method was linear, ranging from 5 to 100 µg/mL, and had a good reproducibility with the RSD below 2%. The contents of ferulic acid in Angelica sinensis and Chuanxiong could be easily determined within 15 min with no pretreatment and no interference. Copyright © 1999 John Wiley & Sons, Ltd.

Ferulic acid in extracts of raw herbs was separated by capillary zone electrophoresis in the buffer solution of 10 mmol/L Na2B4O7. The simple and rapid method was linear, ranging from 5 to 100 µg/mL, and had a good reproducibility with the RSD below 2%. The contents of ferulic acid in Angelica sinensisand Chuanxiongcould be easily determined within 15 min with no pretreatment and no interference. Copyright © 1999 John Wiley & Sons, Ltd.