1. Validated HPLC method for the standardization ofPhyllanthus niruri(herb and commercial extracts) using corilagin as a phytochemical marker
- Author
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Giovani C. C. Bomfim, Clicia R. Pelicia, Vanderlan da Silva Bolzani, Dulce Helena Siqueira Silva, Renata Colombo, Rosilene Cristina Rossetto Burgos, Maria Celia H. Heimberg, Fabiana M. Guimaraes, Andrea N. L. Batista, Helder L. Teles, Geraldo H. Silva, and Alberto José Cavalheiro
- Subjects
Hydrolysable tannin ,Magnetic Resonance Spectroscopy ,Time Factors ,Phyllanthus ,Rutin ,Clinical Biochemistry ,Sensitivity and Specificity ,Biochemistry ,High-performance liquid chromatography ,Analytical Chemistry ,chemistry.chemical_compound ,Column chromatography ,Glucosides ,Gallic Acid ,Drug Discovery ,Medicinal plants ,Molecular Biology ,Chromatography, High Pressure Liquid ,Pharmacology ,chemistry.chemical_classification ,Chromatography ,biology ,Plant Extracts ,Reproducibility of Results ,General Medicine ,biology.organism_classification ,Hydrolyzable Tannins ,Plant Leaves ,chemistry ,Phytochemical ,Corilagin - Abstract
Phyllanthus niruri L., commonly known in Brazil as 'quebra-pedra', has long been used in the treatment of diverse diseases and especially urolithiasis. The therapeutic effects of P. niruri are attributed to various compounds present in the plant, including the hydrolysable tannin corilagin. In the present study, high-performance liquid chromatography (HPLC-/PAD) profiles of leaves and commercial extracts of P. niruri were examined and three compounds, found to be present in all of the samples studied, were isolated by open column chromatography over C18)silica gel followed by preparative HPLC. These compounds were identified by nuclear magnetic resonance as corilagin, rutin and ethyl 3,4,5-trihydroxybenzoate. Corilagin, which has been proposed as a phytochemical marker for P. niruri, was employed as an external standard in the development and validation of a rapid and efficient qualitative and quantitative HPLC assay for the analyte. The method may be applied in the standardization of herbs and phytomedicines commercialized in Brazil as quebra-pedra.
- Published
- 2009
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