Search

Your search keyword '"Zhu, Qing"' showing total 20 results
Start Over Author "Zhu, Qing" Journal biomed research international
20 results on '"Zhu, Qing"'

3. A Prediction Model Based on Noninvasive Indicators to Predict the 8-Year Incidence of Type 2 Diabetes in Patients with Nonalcoholic Fatty Liver Disease: A Population-Based Retrospective Cohort Study

Author

Cai, Xintian, primary, Zhu, Qing, additional, Cao, Yuanyuan, additional, Liu, Shasha, additional, Wang, Mengru, additional, Wu, Ting, additional, Hong, Jing, additional, Ahmat, Ayguzal, additional, Aierken, Xiayire, additional, and Li, Nanfang, additional

Published
2021
Full Text
View/download PDF

18. Effect of Bitter Compounds on the Expression of Bitter Taste Receptor T2R7 Downstream Signaling Effectors in cT2R7/pDisplay-Gα16/gust44/pcDNA3.1 (+) Cells.

Author

Su, Yuan, Jie, Hang, Zhu, Qing, Zhao, Xiaoling, Wang, Yan, Yin, Huadong, Kumar Mishra, Shailendra, and Li, Diyan

Subjects
ANIMAL experimentation, BIRDS, CAMPHOR, CELL receptors, CELLULAR signal transduction, DNA, ERYTHROMYCIN, GENE expression, INGESTION, MEMBRANE proteins, MESSENGER RNA, POLYMERASE chain reaction, POTASSIUM chloride, TASTE buds, HAPLOTYPES, CHLORPHENIRAMINE, PHARMACODYNAMICS
Abstract

Bitterness is an important taste sensation for chickens, which provides useful sensory information for acquisition and selection of diet, and warns them against ingestion of potentially harmful and noxious substances in nature. Bitter taste receptors (T2Rs) mediate the recognition of bitter compounds belonging to a family of proteins known as G-protein coupled receptors. The aim of this study was to identify and evaluate the expression of T2R7 in chicken tongue tissue and construct cT2R7-1 and cT2R7-2-expressing HEK-293T cells to access the expression of PLCβ2 and ITPR3 after exposure with different concentrations of the bitter compounds. Using real-time PCR, we show that the relative expression level of T2R7 mRNA in 5, 1, 0.1, and 10−3 mM of camphor and erythromycin solutions and 5 mM of chlorpheniramine maleate solutions was significantly higher than that in 50 mM KCL solutions. We confirmed that the bitter taste receptor T2R7 and downstream signaling effectors are sensitive to different concentrations of bitter compounds. Moreover, T2R7-1 (corresponding to the unique haplotype of the Tibetan chicken) had higher sensitivity to bitter compounds compared with that of T2R7-2 (corresponding to the unique haplotype of the Jiuyuan black-chicken). These results provide great significance of taste response on dietary intake to improve chicken feeding efficiency in poultry production and have certain reference value for future taste research in other bird species. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

20. Effect of Bitter Compounds on the Expression of Bitter Taste Receptor T2R7 Downstream Signaling Effectors in cT2R7 /pDisplay-G α 16/gust44/pcDNA3.1 (+) Cells.

Author

Su Y, Jie H, Zhu Q, Zhao X, Wang Y, Yin H, Kumar Mishra S, and Li D

Subjects
Animals, Avian Proteins genetics, Chickens, Dose-Response Relationship, Drug, Female, HEK293 Cells, Humans, Inositol 1,4,5-Trisphosphate Receptors biosynthesis, Inositol 1,4,5-Trisphosphate Receptors genetics, Male, Phospholipase C beta biosynthesis, Phospholipase C beta genetics, Receptors, G-Protein-Coupled, Signal Transduction genetics, Avian Proteins biosynthesis, Camphor pharmacology, Chlorpheniramine pharmacology, Erythromycin pharmacology, Gene Expression Regulation drug effects, Signal Transduction drug effects
Abstract

Bitterness is an important taste sensation for chickens, which provides useful sensory information for acquisition and selection of diet, and warns them against ingestion of potentially harmful and noxious substances in nature. Bitter taste receptors (T2Rs) mediate the recognition of bitter compounds belonging to a family of proteins known as G-protein coupled receptors. The aim of this study was to identify and evaluate the expression of T2R7 in chicken tongue tissue and construct cT2R7-1 and cT2R7-2- expressing HEK-293T cells to access the expression of PLCβ2 and ITPR3 after exposure with different concentrations of the bitter compounds. Using real-time PCR, we show that the relative expression level of T2R7 mRNA in 5, 1, 0.1, and 10 -3  mM of camphor and erythromycin solutions and 5 mM of chlorpheniramine maleate solutions was significantly higher than that in 50 mM KCL solutions. We confirmed that the bitter taste receptor T2R7 and downstream signaling effectors are sensitive to different concentrations of bitter compounds. Moreover, T2R7-1 (corresponding to the unique haplotype of the Tibetan chicken) had higher sensitivity to bitter compounds compared with that of T2R7-2 (corresponding to the unique haplotype of the Jiuyuan black-chicken). These results provide great significance of taste response on dietary intake to improve chicken feeding efficiency in poultry production and have certain reference value for future taste research in other bird species., Competing Interests: The authors declare that they have no conflicts of interest., (Copyright © 2019 Yuan Su et al.)

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results