1. Muscle IGF-1-induced skeletal muscle hypertrophy evokes higher insulin sensitivity and carbohydrate use as preferential energy substrate.
- Author
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Christoffolete MA, Silva WJ, Ramos GV, Bento MR, Costa MO, Ribeiro MO, Okamoto MM, Lohmann TH, Machado UF, Musarò A, and Moriscot AS
- Subjects
- Animals, Glucose Transporter Type 4 metabolism, Mice, Mice, Transgenic, Mitochondria metabolism, Muscle Proteins metabolism, Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Peroxisome Proliferator-Activated Receptors metabolism, RNA, Messenger metabolism, Transcription Factors metabolism, Carbohydrate Metabolism physiology, Hypertrophy metabolism, Insulin metabolism, Insulin Resistance physiology, Insulin-Like Growth Factor I metabolism, Muscle, Skeletal metabolism
- Abstract
We characterized the metabolic profile of transgenic mice exhibiting enhanced muscle mass driven by increased mIGF-1 expression (MLC/mIGF-1). As expected, 6-month-old MLC/mIGF-1 mice were heavier than age-matched wild type (WT) mice (37.4 ± 0.3 versus 31.8 ± 0.6 g, resp.). MLC/mIGF-1 mice had higher respiratory quotient when compared to WT (0.9 ± 0.03 versus 0.74 ± 0.02, resp.) suggesting a preference for carbohydrate as the major fuel source. MLC/mIGF-1 mice had a higher rate of glucose disposal when compared to WT (3.25 ± 0.14 versus 2.39 ± 0.03%/min, resp.). The higher disposal rate correlated to ∼ 2-fold higher GLUT4 content in the extensor digitorum longus (EDL) muscle. Analysis of mRNA content for the glycolysis-related gene PFK-1 showed ∼ 3-fold upregulation in MLC/mIGF-1 animals. We also found a 50% downregulation of PGC1α mRNA levels in MLC/mIGF-1 mouse EDL muscle, suggesting less abundant mitochondria in this tissue. We found no difference in the expression of PPARα and PPARβ/δ, suggesting no modulation of key elements in oxidative metabolism. These data together suggest a shift in metabolism towards higher carbohydrate utilization, and that could explain the increased insulin sensitivity of hypertrophied skeletal muscle in MLC/mIGF-1 mice.
- Published
- 2015
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