1. Hepatoprotective and Antioxidant Effect of Bauhinia hookeri Extract against Carbon Tetrachloride-Induced Hepatotoxicity in Mice and Characterization of Its Bioactive Compounds by HPLC-PDA-ESI-MS/MS
- Author
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Abdel-Nasser A. Sabra, Sayed H. Seif el-Din, Naglaa M. El-Lakkany, Olfat Hammam, Eman Al-Sayed, Olli Martiskainen, and Mohamed M. Abdel-Daim
- Subjects
Antioxidant ,Article Subject ,medicine.medical_treatment ,Glutathione reductase ,lcsh:Medicine ,Pharmacology ,digestive system ,General Biochemistry, Genetics and Molecular Biology ,Antioxidants ,Superoxide dismutase ,Lipid peroxidation ,chemistry.chemical_compound ,Mice ,Tandem Mass Spectrometry ,medicine ,Animals ,Carbon Tetrachloride ,Chromatography, High Pressure Liquid ,Liver injury ,chemistry.chemical_classification ,General Immunology and Microbiology ,biology ,Chemistry ,Carbon Tetrachloride Poisoning ,Plant Extracts ,Glutathione peroxidase ,lcsh:R ,General Medicine ,Glutathione ,medicine.disease ,Malondialdehyde ,Oxidative Stress ,Biochemistry ,Bauhinia ,biology.protein ,Lipid Peroxidation ,Chemical and Drug Induced Liver Injury ,Phytotherapy ,Research Article - Abstract
The hepatoprotective and antioxidant activity ofBauhinia hookeriethanol extract (BHE) against CCl4-induced liver injury was investigated in mice. BHE was administered (500 and 1000 mg/kg/day) along with CCl4for 6 weeks. The hepatic marker enzymes: alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were determined in the serum. The antioxidant parameters: glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione transferase (GST), and malondialdehyde (MDA) were estimated in the liver homogenate. BHE treatment significantly inhibited the CCl4-induced increase in ALT (44 and 64%), AST (36 and 46%), ALP (28 and 42%), and MDA (39 and 51%) levels at the tested doses, respectively. Moreover, BHE treatment markedly increased the activity of antioxidant parameters GSH, GPx, GR, GST, and SOD. Histological observations confirmed the strong hepatoprotective activity. These results suggest that a dietary supplement of BHE could exert a beneficial effect against oxidative stress and various liver diseases by enhancing the antioxidant defense status, reducing lipid peroxidation, and protecting against the pathological changes of the liver. The hepatoprotective activity of BHE is mediated, at least in part, by the antioxidant effect of its constituents. The active constituents of BHE were identified by HPLC-PDA-ESI/MS/MS.
- Published
- 2014