1. Proteomic-Based Discovery of Predictive Biomarkers for Drug Therapy Response and Personalized Medicine in Chronic Immune Thrombocytopenia.
- Author
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Gilanchi, Samira, Faranoush, Mohammad, Daskareh, Mahyar, Sadjjadi, Fatemeh Sadat, Zali, Hakimeh, Ghassempour, Alireza, and Rezaei Tavirani, Mostafa
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BIOMARKERS , *BLOOD proteins , *CHRONIC diseases , *ELECTROPHORESIS , *THROMBOPENIC purpura , *INDIVIDUALIZED medicine , *PEROXISOME proliferator-activated receptors , *PROTEOMICS , *TREATMENT effectiveness , *BIOINFORMATICS , *COMPARATIVE studies , *BLOOD platelet activation , *CELLULAR signal transduction , *ENZYME-linked immunosorbent assay , *RESEARCH funding , *MASS spectrometry , *INTESTINAL absorption , *CELL adhesion molecules , *PREDICTION models , *ONTOLOGIES (Information retrieval) - Abstract
Purpose. ITP is the most prevalent autoimmune blood disorder. The lack of predictive biomarkers for therapeutic response is a major challenge for physicians caring of chronic ITP patients. This study is aimed at identifying predictive biomarkers for drug therapy responses. Methods. 2D gel electrophoresis (2-DE) was performed to find differentially expressed proteins. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS) analysis was performed to identify protein spots. The Cytoscape software was employed to visualize and analyze the protein-protein interaction (PPI) network. Then, enzyme-linked immunosorbent assays (ELISA) were used to confirm the results of the proteins detected in the blood. The DAVID online software was used to explore the Gene Ontology and pathways involved in the disease. Results. Three proteins, including APOA1, GC, and TF, were identified as hub-bottlenecks and confirmed by ELISA. Enrichment analysis results showed the importance of several biological processes and pathway, such as the PPAR signaling pathway, complement and coagulation cascades, platelet activation, vitamin digestion and absorption, fat digestion and absorption, cell adhesion molecule binding, and receptor binding. Conclusion and Clinical Relevance. Our results indicate that plasma proteins (APOA1, GC, and TF) can be suitable biomarkers for the prognosis of the response to drug therapy in ITP patients. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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