1. Mechanical Strain Regulates Osteogenic and Adipogenic Differentiation of Bone Marrow Mesenchymal Stem Cells.
- Author
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Li, Runguang, Liang, Liang, Dou, Yonggang, Huang, Zeping, Mo, Huiting, Wang, Yaning, and Yu, Bin
- Subjects
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FAT cells , *HEMATOPOIETIC stem cells , *CELL differentiation , *OBESITY complications , *ACADEMIC medical centers , *ADIPOSE tissues , *ANALYSIS of variance , *BONE growth , *EXERCISE , *IMMUNOHISTOCHEMISTRY , *OSTEOPOROSIS , *POLYMERASE chain reaction , *RESEARCH funding , *WESTERN immunoblotting , *GENOMICS , *PHYSIOLOGIC strain , *REVERSE transcriptase polymerase chain reaction , *DATA analysis software , *DISEASE complications , *PHYSIOLOGY - Abstract
This study examined the effects of mechanical strain on osteogenic and adipogenic differentiation of cultured MSCs by stimulating MSCs cultured in general and adipogenic differentiation media using a mechanical strain device. Markers of osteogenic (Runx2, Osx, and I-collagen) and adipogenic (PPARγ-2, C/EBPα, and lipid droplets) differentiation were examined using real-time PCR, western blot, immunocytochemical, or histochemical stain analyses. Levels of Runx2 and Osx gradually increased in MSC groups in general medium subject to strain stimulation, as compared with in unstrained groups. After adding the stress signal, I-collagen protein levels of expression were obviously promoted in cells in comparison to the controls. The levels of PPARγ-2 and C/EBPα were decreased, and the emergence of lipid droplets was delayed in MSCs groups in adipogenic differentiation medium subject to strain stimulation, as compared with in unstrained groups. Mechanical strain can promote differentiation of MSCs into osteoblasts and can impede differentiation into adipocytes. These results clarify the mechanisms underlying the effects of exercise on bone repair and reconstruction and provide a more adequate scientific basis for the use of exercise therapy in the treatment of obesity and metabolic osteoporosis. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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