1. Bone Marrow-Derived Mesenchymal Stem Cell Potential Regression of Dysplasia Associating Experimental Liver Fibrosis in Albino Rats.
- Author
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Khalifa, Yasmine H., Mourad, Ghada M., Stephanos, Wahid M., Omar, Sahar A., and Mehanna, Radwa A.
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STEM cells , *BONE marrow physiology , *ANIMAL experimentation , *CYTOCHEMISTRY , *ELECTRON microscopy , *FLUORESCENT antibody technique , *GENE expression , *HYDROCARBONS , *IRON compounds , *LIVER , *LIVER function tests , *METALLOPROTEINS , *NANOPARTICLES , *RATS , *TRANSFORMING growth factors-beta , *FIBROSIS , *MATRIX metalloproteinases , *PHYSIOLOGY - Abstract
Objectives. Assessing the therapeutic efficacy of superparamagnetic iron oxide nanoparticles (SPIO) labeled bone marrow-derived mesenchymal stem cells (BM-MSCs) on experimental liver fibrosis and associated dysplasia. Materials and Methods. MSCs were obtained from 10 male Sprague-Dawley rats while 50 female rats were divided into control (CG), liver fibrosis (CCL4, intraperitoneal injection of CCl4 for 8 weeks), and CCL4 rats treated with SPIO-labeled MSCs (MSCs/CCl4) with and without continuing CCL4 injection for another 8 weeks. Assessment included liver histopathology, liver function tests, transmission electron microscopic tracing for homing of SPIO-MSCs, immunofluorescence histochemistry for fibrosis and dysplasia markers (transforming growth factor-beta (TGF-β1), proliferation nuclear antigen (PCNA), glypican 3)), and quantitative gene expression analysis for matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1). Results. SPIO-labeled MSCs were engrafted in the fibrotic liver and the BM/MSCs demonstrated regression for fibrous tissue deposition and inhibition progression of dysplastic changes in the liver of CCl4-treated rats on both the histological and molecular levels. Conclusion. BM-MSCs possess regenerative and antidysplastic potentials. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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