1. Cytotoxicity of cultured macrophages exposed to antimicrobial zinc oxide (ZnO) coatings on nanoporous aluminum oxide membranes.
- Author
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Petrochenko PE, Skoog SA, Zhang Q, Comstock DJ, Elam JW, Goering PL, and Narayan RJ
- Subjects
- Animals, Cell Survival drug effects, Coated Materials, Biocompatible chemistry, Coated Materials, Biocompatible toxicity, Dose-Response Relationship, Drug, Humans, Materials Testing, Membranes, Artificial, Microscopy, Electron, Scanning, Nanostructures chemistry, Nanostructures toxicity, Surface Properties, Zinc Oxide chemistry, Aluminum Oxide chemistry, Macrophages drug effects, Zinc Oxide toxicity
- Abstract
Zinc oxide (ZnO) is a widely used commercial material that is finding use in wound healing applications due to its antimicrobial properties. Our study demonstrates a novel approach for coating ZnO with precise thickness control onto 20 nm and 100 nm pore diameter anodized aluminum oxide using atomic layer deposition (ALD). ZnO was deposited throughout the nanoporous structure of the anodized aluminum oxide membranes. An 8 nm-thick coating of ZnO, previously noted to have antimicrobial properties, was cytotoxic to cultured macrophages. After 48 h, ZnO-coated 20 nm and 100 nm pore anodized aluminum oxide significantly decreased cell viability by ≈65% and 54%, respectively, compared with cells grown on uncoated anodized aluminum oxide membranes and cells grown on tissue culture plates. Pore diameter (20-200 nm) did not influence cell viability.
- Published
- 2013
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