1. The effect of microgrooved culture substrates on calcium cycling of cardiac myocytes derived from human induced pluripotent stem cells
- Author
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Ljudmila Kolker, Patrizia Camelliti, Themistoklis Prodromakis, Thanos Athanasiou, Christopher Rao, Cesare M. Terracciano, Arun Sridhar, Tatiana Trantidou, Magdi H. Yacoub, Ara Darzi, Umar A.R. Chaudhry, Sian E. Harding, Claire Weekes, National Institute for Health Research, and British Heart Foundation
- Subjects
DYNAMICS ,Technology ,TRIADIN ,02 engineering and technology ,Stem cells ,Cardiac tissue engineering ,Sarcomere ,CARDIOMYOCYTES ,Tissue culture ,Engineering ,Myocyte ,Micropatterning ,Myocytes, Cardiac ,Induced pluripotent stem cell ,Materials Science, Biomaterials ,ARCHITECTURE ,0303 health sciences ,biology ,MUSCLE ,021001 nanoscience & nanotechnology ,NETWORKS ,Cell biology ,Electrophysiology ,Mechanics of Materials ,Calcium cycling ,Stem cell ,0210 nano-technology ,Materials science ,Materials Science ,MODELS ,Induced Pluripotent Stem Cells ,Biomedical Engineering ,Biophysics ,Bioengineering ,LONG-QT SYNDROME ,Article ,Biomaterials ,03 medical and health sciences ,REGENERATION ,Humans ,Dimethylpolysiloxanes ,Engineering, Biomedical ,030304 developmental biology ,Science & Technology ,Polydimethylsiloxane ,Endoplasmic reticulum ,REPOLARIZATION ,Models, Theoretical ,Fibronectin ,biology.protein ,Ceramics and Composites ,Biomedical engineering - Abstract
Induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM) have been widely proposed as in vitro models of myocardial physiology and disease. A significant obstacle, however, is their immature phenotype. We hypothesised that Ca2+ cycling of iPSC-CM is influenced by culture conditions and can be manipulated to obtain a more mature cellular behaviour. To test this hypothesis we seeded iPSC-CM onto fibronectin coated microgrooved polydimethylsiloxane (PDMS) scaffolds fabricated using photolithography, or onto unstructured PDMS membrane. After two weeks in culture, the structure and function of iPSC-CM were studied. PDMS microgrooved culture substrates brought about cellular alignment (p
- Published
- 2012