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Start Over Author "Khademhosseini, Ali" Journal biomaterials
231 results on '"Khademhosseini, Ali"'

8. Tri-layered elastomeric scaffolds for engineering heart valve leaflets

Author

Masoumi, Nafiseh, Annabi, Nasim, Assmann, Alexander, Larson, Benjamin L, Hjortnaes, Jesper, Alemdar, Neslihan, Kharaziha, Mahshid, Manning, Keefe B, Mayer, John E, and Khademhosseini, Ali

Subjects
Biotechnology, Regenerative Medicine, Stem Cell Research - Nonembryonic - Non-Human, Bioengineering, Cardiovascular, Stem Cell Research, Transplantation, 5.2 Cellular and gene therapies, Development of treatments and therapeutic interventions, Musculoskeletal, Animals, Anisotropy, Aortic Valve, Elastomers, Heart Valve Prosthesis, Humans, In Vitro Techniques, Materials Testing, Microscopy, Electron, Scanning, Polymers, Pulmonary Valve, Sheep, Tissue Engineering, Tissue Scaffolds, Microfabricated elastomer, Electrospinning, Biodegradable scaffold, Anisotropic mechanical properties, Heart valve tissue engineering
Abstract

Tissue engineered heart valves (TEHVs) that can grow and remodel have the potential to serve as permanent replacements of the current non-viable prosthetic valves particularly for pediatric patients. A major challenge in designing functional TEHVs is to mimic both structural and anisotropic mechanical characteristics of the native valve leaflets. To establish a more biomimetic model of TEHV, we fabricated tri-layered scaffolds by combining electrospinning and microfabrication techniques. These constructs were fabricated by assembling microfabricated poly(glycerol sebacate) (PGS) and fibrous PGS/poly(caprolactone) (PCL) electrospun sheets to develop elastic scaffolds with tunable anisotropic mechanical properties similar to the mechanical characteristics of the native heart valves. The engineered scaffolds supported the growth of valvular interstitial cells (VICs) and mesenchymal stem cells (MSCs) within the 3D structure and promoted the deposition of heart valve extracellular matrix (ECM). MSCs were also organized and aligned along the anisotropic axes of the engineered tri-layered scaffolds. In addition, the fabricated constructs opened and closed properly in an ex vivo model of porcine heart valve leaflet tissue replacement. The engineered tri-layered scaffolds have the potential for successful translation towards TEHV replacements.

Published
2014

9. Tough and flexible CNT–polymeric hybrid scaffolds for engineering cardiac constructs

Author

Kharaziha, Mahshid, Shin, Su Ryon, Nikkhah, Mehdi, Topkaya, Seda Nur, Masoumi, Nafiseh, Annabi, Nasim, Dokmeci, Mehmet R, and Khademhosseini, Ali

Subjects
Heart Disease, Nanotechnology, Cardiovascular, Regenerative Medicine, Bioengineering, Development of treatments and therapeutic interventions, 5.2 Cellular and gene therapies, Animals, Biocompatible Materials, Cells, Cultured, Decanoates, Electric Conductivity, Gelatin, Glycerol, Myocardium, Myocytes, Cardiac, Nanofibers, Nanotubes, Carbon, Polymers, Rats, Sprague-Dawley, Tissue Engineering, Tissue Scaffolds, Cardiac tissue engineering, Scaffold, Cardiomyocyte, Poly(glycerol sebacate):gelatin, Carbon Nanotubes
Abstract

In the past few years, a considerable amount of effort has been devoted toward the development of biomimetic scaffolds for cardiac tissue engineering. However, most of the previous scaffolds have been electrically insulating or lacked the structural and mechanical robustness to engineer cardiac tissue constructs with suitable electrophysiological functions. Here, we developed tough and flexible hybrid scaffolds with enhanced electrical properties composed of carbon nanotubes (CNTs) embedded aligned poly(glycerol sebacate):gelatin (PG) electrospun nanofibers. Incorporation of varying concentrations of CNTs from 0 to 1.5% within the PG nanofibrous scaffolds (CNT-PG scaffolds) notably enhanced fiber alignment and improved the electrical conductivity and toughness of the scaffolds while maintaining the viability, retention, alignment, and contractile activities of cardiomyocytes (CMs) seeded on the scaffolds. The resulting CNT-PG scaffolds resulted in stronger spontaneous and synchronous beating behavior (3.5-fold lower excitation threshold and 2.8-fold higher maximum capture rate) compared to those cultured on PG scaffold. Overall, our findings demonstrated that aligned CNT-PG scaffold exhibited superior mechanical properties with enhanced CM beating properties. It is envisioned that the proposed hybrid scaffolds can be useful for generating cardiac tissue constructs with improved organization and maturation.

Published
2014

12. PGS:Gelatin nanofibrous scaffolds with tunable mechanical and structural properties for engineering cardiac tissues.

Author

Kharaziha, Mahshid, Nikkhah, Mehdi, Shin, Su-Ryon, Annabi, Nasim, Masoumi, Nafiseh, Gaharwar, Akhilesh K, Camci-Unal, Gulden, and Khademhosseini, Ali

Subjects
Myocardium, Cells, Cultured, Fibroblasts, Myocytes, Cardiac, Animals, Rats, Rats, Sprague-Dawley, Glycerol, Polymers, Decanoates, Gelatin, Tissue Engineering, Cell Adhesion, Cell Proliferation, Tissue Scaffolds, Nanofibers, Scaffold, Nanofibrous, poly(glycerol sebacate):gelatin, Cardiac cells, Tissue engineering, Cells, Cultured, Myocytes, Cardiac, Sprague-Dawley, Biomedical Engineering
Abstract

A significant challenge in cardiac tissue engineering is the development of biomimetic grafts that can potentially promote myocardial repair and regeneration. A number of approaches have used engineered scaffolds to mimic the architecture of the native myocardium tissue and precisely regulate cardiac cell functions. However, previous attempts have not been able to simultaneously recapitulate chemical, mechanical, and structural properties of the myocardial extracellular matrix (ECM). In this study, we utilized an electrospinning approach to fabricate elastomeric biodegradable poly(glycerol sebacate) (PGS):gelatin nanofibrous scaffolds with a wide range of chemical composition, stiffness and anisotropy. Our findings demonstrated that through incorporation of PGS, it is possible to create nanofibrous scaffolds with well-defined anisotropy that mimic the left ventricular myocardium architecture. Furthermore, we studied attachment, proliferation, differentiation and alignment of neonatal rat cardiac fibroblast cells (CFs) as well as protein expression, alignment, and contractile function of cardiomyocyte (CMs) on PGS:gelatin scaffolds with variable amount of PGS. Notably, aligned nanofibrous scaffold, consisting of 33 wt. % PGS, induced optimal synchronous contractions of CMs while significantly enhanced cellular alignment. Overall, our study suggests that the aligned nanofibrous PGS:gelatin scaffold support cardiac cell organization, phenotype and contraction and could potentially be used to develop clinically relevant constructs for cardiac tissue engineering.

Published
2013

13. A highly adhesive and naturally derived sealant

Author

Assmann, Alexander, Vegh, Andrea, Ghasemi-Rad, Mohammad, Bagherifard, Sara, Cheng, George, Sani, Ehsan Shirzaei, Ruiz-Esparza, Guillermo U., Noshadi, Iman, Lassaletta, Antonio D., Gangadharan, Sidhu, Tamayol, Ali, Khademhosseini, Ali, and Annabi, Nasim

Published
2017
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