1. Novel PCL-based honeycomb scaffolds as drug delivery systems for rhBMP-2
- Author
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Swee Hin Teoh, Dietmar W. Hutmacher, Bina Rai, Tong Cao, and K.H. Ho
- Subjects
Calcium Phosphates ,Materials science ,Scanning electron microscope ,Sodium ,Polyesters ,Biophysics ,Drug Evaluation, Preclinical ,chemistry.chemical_element ,Bone Morphogenetic Protein 2 ,Bioengineering ,Fibrin Tissue Adhesive ,Biomaterials ,Diffusion ,chemistry.chemical_compound ,Coated Materials, Biocompatible ,BMP ,Bone tissue engineering ,Drug delivery ,Fibrin ,Polycaprolactone ,Transforming Growth Factor beta ,Materials Testing ,Bone regeneration ,Polyacrylamide gel electrophoresis ,Drug Implants ,Sealant ,equipment and supplies ,090300 BIOMEDICAL ENGINEERING ,090400 CHEMICAL ENGINEERING ,Body Fluids ,Drug Combinations ,chemistry ,Mechanics of Materials ,Bone Morphogenetic Proteins ,Bone Substitutes ,Ceramics and Composites ,Alkaline phosphatase ,069900 OTHER BIOLOGICAL SCIENCES ,Biomedical engineering - Abstract
This study investigated a novel drug delivery system (DDS), consisting of polycaprolactone (PCL) or polycaprolactone 20% tricalcium phosphate (PCL-TCP) biodegradable scaffolds, fibrin Tisseel sealant and recombinant bone morphogenetic protein-2 (rhBMP-2) for bone regeneration. PCL and PCL-TCP-fibrin composites displayed a loading efficiency of 70% and 43%, respectively. Fluorescence and scanning electron microscopy revealed sparse clumps of rhBMP-2 particles, non-uniformly distributed on the rods' surface of PCL-fibrin composites. In contrast, individual rhBMP-2 particles were evident and uniformly distributed on the rods' surface of the PCL-TCP-fibrin composites. PCL-fibrin composites loaded with 10 and 20 microg/ml rhBMP-2 demonstrated a triphasic release profile as quantified by an enzyme-linked immunosorbent assay (ELISA). This consisted of burst releases at 2 h, and days 7 and 16. A biphasic release profile was observed for PCL-TCP-fibrin composites loaded with 10 microg/ml rhBMP-2, consisting of burst releases at 2 h and day 14. PCL-TCP-fibrin composites loaded with 20 microg/ml rhBMP-2 showed a tri-phasic release profile, consisting of burst releases at 2 h, and days 10 and 21. We conclude that the addition of TCP caused a delay in rhBMP-2 release. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and alkaline phosphatase assay verified the stability and bioactivity of eluted rhBMP-2 at all time points.
- Published
- 2004