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Start Over Author "Healy, Kevin E." Journal biomaterials
16 results on '"Healy, Kevin E."'

3. Three-dimensional filamentous human diseased cardiac tissue model

Author

Ma, Zhen, Koo, Sangmo, Finnegan, Micaela A, Loskill, Peter, Huebsch, Nathaniel, Marks, Natalie C, Conklin, Bruce R, Grigoropoulos, Costas P, and Healy, Kevin E

Subjects
Biomedical and Clinical Sciences, Engineering, Biomedical Engineering, Heart Disease, Stem Cell Research, Stem Cell Research - Induced Pluripotent Stem Cell, Cardiovascular, Stem Cell Research - Induced Pluripotent Stem Cell - Human, 2.1 Biological and endogenous factors, Aetiology, Case-Control Studies, Cell Differentiation, Drug Evaluation, Preclinical, Flow Cytometry, Humans, Induced Pluripotent Stem Cells, Long QT Syndrome, Microscopy, Atomic Force, Microscopy, Electron, Scanning, Models, Biological, Real-Time Polymerase Chain Reaction, Induced pluripotent stem cells, Cardiac disease model, Two-photon initiated polymerization, Long QT syndrome, Cardiac contractility, Drug testing
Abstract

A human in vitro cardiac tissue model would be a significant advancement for understanding, studying, and developing new strategies for treating cardiac arrhythmias and related cardiovascular diseases. We developed an in vitro model of three-dimensional (3D) human cardiac tissue by populating synthetic filamentous matrices with cardiomyocytes derived from healthy wild-type volunteer (WT) and patient-specific long QT syndrome type 3 (LQT3) induced pluripotent stem cells (iPS-CMs) to mimic the condensed and aligned human ventricular myocardium. Using such a highly controllable cardiac model, we studied the contractility malfunctions associated with the electrophysiological consequences of LQT3 and their response to a panel of drugs. By varying the stiffness of filamentous matrices, LQT3 iPS-CMs exhibited different level of contractility abnormality and susceptibility to drug-induced cardiotoxicity.

Published
2014

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