1. Pyrrolopyrrole aza-BODIPY-based NIR-II fluorophores for in vivo dynamic vascular dysfunction visualization of vascular-targeted photodynamic therapy.
- Author
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Bian, Shuaishuai, Zheng, Xiuli, Liu, Weimin, Li, Jihao, Gao, Zekun, Ren, Haohui, Zhang, Wenjun, Lee, Chun-Sing, and Wang, Pengfei
- Subjects
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PHOTODYNAMIC therapy , *FLUOROPHORES , *BLOOD circulation , *BLOOD flow , *FLOW velocity , *COLLATERAL circulation - Abstract
Real-time monitoring vascular responses is crucial for evaluating the therapeutic effects of vascular-targeted photodynamic therapy (V-PDT). Herein, we developed a highly-stable and bright aggregation induced emission (AIE) fluorophore (PTPE3 NP) for dynamic fluorescence (FL) imaging of vascular dysfunction beyond 1300 nm window during V-PDT. The superior brightness (ϵ max Φ f>1000 nm ≈ 180.05 M−1 cm−1) and high resolution of PTPE3 NP affords not only high-clarity images of whole-body and local vasculature (hindlimbs, mesentery, and tumor) but also high-speed video imaging for tracking blood circulation process. By virtue of the NPs' prolonged blood circulation time (t 1/2 ≈ 86.5 min) and excellent photo/chemical (pH, RONS) stability, mesenteric and tumor vascular dysfunction (thrombosis formation, vessel occlusion, and hemorrhage) can be successfully visualized during V-PDT by FL imaging for the first time. Furthermore, the reduction of blood flow velocity (BFV) can be monitored in real time for precisely evaluating efficacy of V-PDT. These provide a powerful approach for assessing vascular responses during V-PDT and promote the development of advanced fluorophores for biological imaging. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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