1. Xyloglucan-Derivatized Films for the Culture of Adherent Cells and Their Thermocontrolled Detachment: A Promising Alternative to Cells Sensitive to Protease Treatment
- Author
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Michel Bessodes, Guylaine Ducouret, Daniel Scherman, Amanda K. A. Silva, Otto-Wilhelm Merten, Cyrille Richard, Généthon, Unité de pharmacologie chimique et génétique et d'imagerie (UPCGI - UMR 8151 / UMR_S 1022 ), Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC), Sciences et Ingénierie de la Matière Molle (SIMM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), GENETHON 3, Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL (ENSCP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-PSL Research University (PSL)-ESPCI ParisTech-Université Pierre et Marie Curie - Paris 6 (UPMC), Unité de pharmacologie chimique et génétique et d'imagerie ( UPCGI - UMR 8151 / UMR_S 1022 ), Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL ( ENSCP ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Sciences et Ingénierie de la Matière Molle ( SIMM ), Centre National de la Recherche Scientifique ( CNRS ) -PSL Research University ( PSL ) -ESPCI ParisTech-Université Pierre et Marie Curie - Paris 6 ( UPMC ), Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Materials science ,Polymers and Plastics ,Polymers ,Cell Culture Techniques ,Bioengineering ,Polymer architecture ,02 engineering and technology ,SPINODAL DECOMPOSITION ,Phase Transition ,PHASE-BEHAVIOR ,Cell Line ,Biomaterials ,Surface tension ,03 medical and health sciences ,chemistry.chemical_compound ,Polymer chemistry ,Cell Adhesion ,NEUTRON REFLECTION ,Materials Chemistry ,Bancroft rule ,Copolymer ,Glucans ,Cell Proliferation ,030304 developmental biology ,INTERFACIAL-TENSION ,ELECTRON-MICROSCOPY ,chemistry.chemical_classification ,0303 health sciences ,Molar mass ,AMPHIPHILIC POLYELECTROLYTE ,Galactose ,Polymer ,beta-Galactosidase ,021001 nanoscience & nanotechnology ,BINARY-FLUID ,DIBLOCK COPOLYMERS ,Monomer ,chemistry ,Chemical engineering ,Emulsion ,LIGHT-TRIGGERED CONTROL ,Xylans ,[ PHYS.COND.CM-SCM ] Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft] ,0210 nano-technology ,[PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft] ,HYDROPHOBICALLY-MODIFIED POLYELECTROLYTES ,Peptide Hydrolases - Abstract
International audience; We investigated emulsions of water and toluene stabilized by (co) polymers consisting of styrene (S) and 2-(dimethylamino) ethyl methacrylate (DMAEMA) monomer units with different compositions and structures such as a PDMAEMA homopolymer, a P(S-co-DMAEMA) random copolymer and various PS-b-PDMAEMA and PS-b-(S-co-DMAEMA) block copolymers. The model system is used to study the fundamental conditions under which the different kinds of polymer-stabilized emulsions (direct oil in water, inverse water in oil and multiple emulsions) are stabilized or destabilized by pH change (at constant temperature). Polymer properties like chain conformation at the toluene-water interface as probed by SANS and neutron reflectivity at the liquid-liquid interface, the oil-water partitioning of the polymer chains (Bancroft's rule of thumb) as determined by UV spectroscopy and interfacial tensions measured by the rising and spinning drop techniques are determined. Overall, results evidence that the curvature sign, as defined by positive and negative values as the chain segments occupy preferentially the water and toluene sides of the interface respectively, reliably predicts the emulsion kind. In contrast, the Bancroft rule failed at foreseeing the emulsion type. In the region of near zero curvature the crossover from direct to inverse emulsions occurs through the formation of either unstable coexisting direct and inverse emulsions (i) or multiple emulsions (ii). The high compact adsorption of the chains at the interface as shown by low interfacial tension values does not allow to discriminate between both cases. However, the toluene-water partitioning of the polymeric emulsifier is still a key factor driving the formation of (i) or (ii) emulsions. Interestingly, the stabilization of the multiple emulsions can be tuned to a large extent as the toluene-water polymer partitioning can be adjusted using quite a large number of physico-chemical parameters linked to polymer architecture like diblock length ratio or polymer total molar mass, for example. Moreover, we show that monitoring the oil-water partitioning aspect of the emulsion system can also be used to lower the interfacial tension at low pH to values slightly higher than 0.01 mN m(-1), irrespective of the curvature sign.
- Published
- 2013