1. Effect of insulin on glucose/oxygen and lactate/oxygen quotients across the hindlimb of fetal lambs.
- Author
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Wilkening RB, Molina RD, Battaglia FC, and Meschia G
- Subjects
- Animals, Fetal Blood drug effects, Hindlimb blood supply, Insulin blood, Sheep, Blood Glucose metabolism, Fetal Blood metabolism, Insulin pharmacology, Lactates blood, Oxygen blood
- Abstract
In order to determine whether insulin stimulates glucose uptake by the hindlimb tissue of the fetal lamb, we performed 7 paired, euglycemic glucose 'clamp' experiments in 7 chronically prepared fetal sheep. Four sample sets for oxygen content, glucose and lactate concentration were drawn from the external iliac artery and vein during a control period and repeated during a euglycemic, hyperinsulinemic 'clamp' period. Insulin was infused at constant rate (4 mU/min/kg) and the fetal arterial glucose concentration was maintained by variable glucose infusion. The glucose/oxygen quotient increased significantly: 1.05 +/- 0.07 (control) versus 2.02 +/- 0.16 (hyperinsulinemia). There was no change in lactate/oxygen quotient. The data demonstrate that with maximal insulin stimulation, fetal hindlimb tissues increase glucose uptake by 92% with no large change in lactate production, indicating a net accumulation of glucose carbon under these experimental conditions.
- Published
- 1987
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