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Food quality and quantity, as well as temperature, are all factors that are expected to affect rates of development, and are likely to be affected by expected climatic change. We tested the effect of a mixed diet versus a single-food diet on metamorphic competence in the emerging model species Crepidula fornicata. We then compared our results with other published studies on this species that examined time to metamorphic competence across a range of food concentrations and rearing temperatures. Ours was the only study to test the effects of single food versus a mixed diet on metamorphic competence for this species. Diet composition did not affect metamorphic competence or survivorship. Comparing results across studies, we found that the shortest time to metamorphic competence was typically found when the food availability per larva was the greatest, independent of rearing temperature. Unfortunately, some published studies did not include important metadata needed for comparison with other studies; these data included larval rearing density, food density, frequency of feeding, and rearing temperature. Mortality rates were not always reported and when reported were often measured in different ways, preventing comparison. Such metadata are essential for comparisons among studies as well as among taxa, and for the determination of generalizable patterns and evolutionary trends. Increased reporting of all such metadata is essential if we are to use scientific studies performed to their fullest potential. [ABSTRACT FROM AUTHOR]

Adelphophagic development, where embryos consume sibling embryos or nurse eggs, is particularly common in marine caenogastropods and some families of polychaetes. When exogenous nutrition is provided before hatching, egg size and hatching size can be uncoupled, but advantages and constraints of adelphophagic development compared to development from large eggs are unknown. Here we examine temperature-mediated plasticity in offspring size, brooding duration, and fecundity in the adelphophagic marine gastropod Crepidula cf. onyx. We use these data combined with previously published data on two planktotrophic Crepidula and two Crepidula species that develop from large eggs to test hypotheses about the consequences of adelphophagic development and patterns of variation in offspring size. In Crepidula cf. onyx, egg size shows no significant effect of temperature. Hatching size is significantly larger at 28 °C than at 23°C but proceeds from fewer eggs per capsule at 28 °C. Hatching size is therefore decoupled from both egg size and the number of eggs per capsule. Although development is faster at the higher temperature, broods are produced roughly every 26-27 days at both temperatures. Increased rate of development has been cited as a potential advantage of adelphophagic development in muricids, but the adelphophagic C. cf. onyx did not develop more quickly than C. atrasolea or C. ustulatulina, species that produce similarly sized hatchlings from large eggs. Comparisons across calyptraeid species support the role of adelphophagy in increasing variance in offspring size. This increased variability is primarily expressed within broods or among broods from the same female, not among females. [ABSTRACT FROM AUTHOR]

Recent developmental and genomic research focused on "slipper snails" in the genus Crepidula has positioned Crepidula fornicata as a de facto model system for lophotrochozoan development. Here we review recent developments, as well as earlier reports demonstrating the widespread use of this system in studies of development and life history. Recent studies have resulted in a well-resolved fate map of embryonic cell lineage, documented mechanisms for axis determination and D quadrant specification, preliminary gene expression patterns, and the successful application of loss- and gain-of-function assays. The recent development of expressed sequence tags and preliminary genomics work will promote the use of this system, particularly in the area of developmental biology. A wealth of comparative information on phylogenetic relationships, variation in mode of development within the family, and numerous studies on larval biology and metamorphosis, primarily in Crepidula fornicata, make these snails a powerful tool for studies of the evolution of the mechanisms of development in the Mollusca and Lophotrochozoa. By bringing a review of the current state of knowledge of Crepidula life histories and development together with some detailed experimental methods, we hope to encourage further use of this system in various fields of investigation. [ABSTRACT FROM AUTHOR]

The size-advantage model and sex-allocation theory are frequently invoked to explain the evolution and maintenance of sequential hermaphroditism in many taxa. A test of current theory requires quantitative estimates of reproductive success and knowledge of the relationship between reproduction and size for each gender. Reproductive success can be difficult to measure. In species where polyandry occurs, it can be quantified only by determining paternity of offspring. We employed microsatellite loci to establish paternity for 12 families of Crepidula fornicata, where a family is defined as a single female, her brood, and the males stacked on top of her. Genetic data were analyzed and paternity was assigned to a single potential father for more than 83% of the offspring tested. Estimates of reproductive success revealed that one male within the family fathered the majority of offspring and that he was usually the largest male and the one closest to the brooding female. The dominant male's success also tended to decrease as the number of mature males within the family increased. Our results suggest that sperm competition could be a driving force in determining male reproductive success and the timing of sex change in C. fornicata. [ABSTRACT FROM AUTHOR]

This paper concerns the role of nitric oxide (NO) in controlling metamorphosis in the marine gastropod Crepidula fornicata. Metamorphosis was stimulated by the nitric oxide synthase (NOS) inhibitors AGH (aminoguanidine hemisulfate) and SMIS (S-methylisothiourea sulfate) at concentrations of about 100-1000 µmol l-1 and 50-200 µmol l-1, respectively. Metamorphosis was not, however, induced by the NOS inhibitor L-NAME (L-Ng-nitroarginine methyl ester) at even the highest concentration tested, 500 p, mol l-1. Moreover, pre-incubation with L-NAME at 20 and 80 µmol l-1 did not increase the sensitivity of competent larvae to excess K+, a potent inducer of metamorphosis in this species; we suggest that either L-NAME is ineffective in suppressing NO production in larvae of C. fornicata, or that it works only on the constitutive isoform of the enzyme. In contrast, metamorphosis was potentiated by the guanylate cyclase inhibitor ODQ (1H-[1,2,4]oxadiazolo[4,3, -a]quinoxalin-l-one) in response to a natural metamorphic inducer derived from conspecific adults. Because NO typically stimulates cGMP production through the activation of soluble guanylate cyclase, this result supports the hypothesis that NO acts as an endogenous inhibitor of metamorphosis in C. fornicata. The expression of NOS, shown by immunohistochemical techniques, was detected in the apical ganglion of young larvae but not in older larvae, further supporting the hypothesis that metamorphosis in C. fornicata is made possible by declines in the endogenous concentration of NO during development. [ABSTRACT FROM AUTHOR]

Examines the structure and function of the embryonic velum of two closely related species of Crepidula with different modes of development. Evolution of direct development from the putative ancestral state of development with planktotrophic larvae in marine invertebrates; Development of embryos of both Crepidula dilatata and Crepidula fecunda from eggs; Embryonic morphology of the direct-developing Crepidula dilatata.

Presents a study which measured shell length of Crepidula fornicata and prosomal width of Limulus polyphemus in Pleasant Bay in Chatham, Massachusetts. Difficulties in estimating horseshoe crab age; Analysis of cohorts.