Wang, Minqiu, Yuan, Meiling, Li, Xinyu, Wu, Xingtong, Ueno, Saneyoshi, Cai, Mengying, Tsumura, Yoshihiko, and Wen, Yafeng
Cryptomeria japonica var. sinensis (Cupressaceae) is an important plantation species that is native to China. Based on the complete chloroplast genome sequence, we developed 11 novel polymorphic chloroplast microsatellite markers and assessed their genetic characterization among three populations. A total of 30 haplotypes were detected in the three populations, and the number of haplotypes per population ranged from 7 to 15. The number of private haplotypes detected in the three populations ranged from 7 to 12. The effective number of haplotypes ranged from 2.90 to 10.37 with an average of 5.84 and the haplotypic richness (Rh) ranged from 6.00 to 12.54. Genetic diversity (He) ranged from 0.69 to 0.95 with an average of 0.81. The number of alleles per locus and population ranged from 1 to 4, and the effective number of alleles ranged from 1.00 to 3.21. In addition, these novel markers were tested by cross-species amplification in four Cupressaceae species. Except for the Cf_cp_12 locus, all polymorphic loci can successfully amplify in four Cupressaceae species: Glyptostrobus pensilis, Taiwania cryptomerioides, C. japonica, and Cunninghamin lanceolata. The chloroplast microsatellite markers reported here will facilitate the characterization of the population genetic and phylogeographic analyses of C. japonica var. sinensis effectively. [ABSTRACT FROM AUTHOR]