1. Observation on the Rotylenchus brevicaudatus Colbran, 1962 from Botswana, with the first SEM of the species.
- Author
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Shokoohi, Ebrahim and Abolafia, Joaquín
- Subjects
RHIZOSPHERE ,SPECIES ,SOIL sampling ,RECOMBINANT DNA ,AUSTRALIANS - Abstract
Rotylenchus brevicaudatus was identified morphologically from soil samples collected around the rhizosphere of wild grass in the North West Province of Botswana. This species is characterized by a rounded lip region bearing four incisures, with slight depression. The basal annuli are divided into six to seven blocks in the ventral view. Tail is rounded with 8–14 μm length, stylet is 21–23 μm in length, spicules 25–26 μm, and gubernaculum 9–11 μm long. The nblast analysis based on the D2-D3 segment of 28S rDNA revealed 96% identity of the Botswanan populations of R. brevicaudatus to the Australian R. brevicaudatus (JX015419, JX015420). The phylogenetic analysis put the Botswanan and Australian population of R. brevicaudatus with a 1.00 posterior probability support. This is the first record of R. brevicaudatus in Botswana. In addition, SEM photographs of R. brevicaudatus are provided for the first time. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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