1. In silico identification of putative metal binding motifs.
- Author
-
Thilakaraj R, Raghunathan K, Anishetty S, and Pennathur G
- Subjects
- Amino Acid Motifs, Amino Acid Sequence, Binding Sites, Databases, Protein, Molecular Sequence Data, Protein Binding, Structure-Activity Relationship, Bacterial Proteins chemistry, Metalloproteins chemistry, Metals chemistry, Sequence Alignment methods, Sequence Analysis, Protein methods
- Abstract
Metal ion binding domains are found in proteins that mediate transport, buffering or detoxification of metal ions. In this study, we have performed an in silico analysis of metal binding proteins and have identified putative metal binding motifs for the ions of cadmium, cobalt, zinc, arsenic, mercury, magnesium, manganese, molybdenum and nickel. A pattern search against the UniProtKB/Swiss-Prot and UniProtKB/TrEMBL databases yielded true positives in each case showing the high-specificity of the motifs. Motifs were also validated against PDB structures and site directed mutagenesis studies.
- Published
- 2007
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