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Your search keyword '"Terpinen-4-ol"' showing total 5 results
Start Over Descriptor "Terpinen-4-ol" Journal biofouling
5 results on '"Terpinen-4-ol"'

1. Terpinen-4-ol and carvacrol affect multi-species biofilm composition.

Author

Maquera Huacho, Patricia Milagros, Rodriguez Herrero, Esteban, Verspecht, Tim, Pauwels, Martine, Marcantonio, Elcio, Palomari Spolidorio, Denise Madalena, and Teughels, Wim

Subjects
CARVACROL, GENTIAN violet, EPITHELIAL cells, CELL survival, BIOFILMS, ORAL examinations (Education)
Abstract

The aim of this study was to investigate the cytotoxic activity and inhibitory effect of terpinen-4-ol (T4ol) and carvacrol against single- and multi-species biofilms. The toxicity of each compound was tested on oral keratinocytes and evaluated by XTT assay. Inhibition and eradication of single-species biofilms were analyzed by crystal violet assay and the effect on multi-species biofilm composition was evaluated by qPCR. T4ol and carvacrol did not affect the epithelial cell viability, in contrast to chlorhexidine, which showed a high cytotoxic effect. Inhibition and eradication of single-species biofilms treated with T4ol and carvacrol were observed. The same inhibitory effect was observed for multi-species biofilms, especially on periodontal pathogens. In conclusion, specific concentrations of T4ol and carvacrol without toxicity towards the epithelial cells reduced the numbers of periodontal pathogens in single- and multi-species biofilms. [ABSTRACT FROM AUTHOR]

Published
2019
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2. Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression.

Author

Bordini, Ester Alves Ferreira, Tonon, Caroline Coradi, Francisconi, Renata Serignoli, Magalhães, Fernando Augusto Cintra, Huacho, Patrícia Milagros Maquera, Bedran, Telma Lombardo, Pratavieira, Sebastião, Spolidorio, Luis Carlos, and Spolidorio, Denise Palomari

Subjects
LACTOBACILLUS acidophilus, ANTI-infective agents, GENE expression, DRUG activation, CONFOCAL microscopy
Abstract

This study evaluated the antibacterial activity of terpinen-4-ol against Streptococcus mutans and Lactobacillus acidophilus and its influence on gbpA (S. mutans) and slpA (L. acidophilus) gene expression. As measured by XTT assay, the concentrations of terpinen-4-ol that effectively inhibited the biofilm were 0.24% and 0.95% for S. mutans and L. acidophilus, respectively. Confocal microscopy revealed the presence of a biofilm attached to the enamel and dentin block surfaces with significant terpinen-4-ol effects against these microorganisms. The expression of the gbpA and slpA genes involved in adherence and biofilm formation was investigated using RT-PCR. Expression of these genes decreased after 15 min with 0.24% and 0.95% terpinen-4-ol in S. mutans and L. acidophilus, respectively. These findings demonstrate the antimicrobial activity of terpinen-4-ol and its ability to modulate the expression of gbpA and slpA genes, emphasizing the therapeutic capacity of terpinen-4-ol as an alternative to inhibit adherence in biofilm. [ABSTRACT FROM AUTHOR]

Published
2018
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3. In vitro antibacterial and cytotoxic activities of carvacrol and terpinen-4-ol against biofilm formation on titanium implant surfaces.

Author

Maquera-Huacho, Patricia Milagros, Tonon, Caroline Coradi, Correia, Marilia Ferreira, Francisconi, Renata Serignoli, Bordini, Ester Alves Ferreira, Marcantonio, Élcio, and Spolidorio, Denise Madalena Palomari

Subjects
CARVACROL, ANTIBACTERIAL agents, ANTINEOPLASTIC agents, CONFOCAL microscopy, FUSOBACTERIUM
Abstract

This study evaluated the antibacterial properties of carvacrol and terpinen-4-ol against Porphyromonas gingivalis and Fusobacterium nucleatum and its cytotoxic effects on fibroblast cells. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were examined. The minimum biofilm inhibition concentration (MBIC) was evaluated by XTT assay. Biofilm decontamination on titanium surfaces was quantified (CFU ml−1), evaluated by confocal laser scanning microscopy (CLSM) and cytotoxic activity by MTT. The MIC and MBC for carvacrol were 0.007% and 0.002% for P. gingivalis and F. nucleatum, and 0.06% for terpinen-4-ol for both microorganisms. The MBIC for carvacrol was 0.03% and 0.06% for P. gingivalis and F. nucleatum, and for terpinen-4-ol was 0.06% and 0.24%. The results indicated anti-biofilm activity using carvacrol (0.26%, 0.06%) and terpinen-4-ol (0.95%, 0.24%) and showed cytotoxic activity similar to chlorohexidine (CHX). However, terpinen-4-ol (0.24%) showed higher cell viability than other treatments. Carvacrol and terpinen-4-ol showed antibacterial activity in respect of reducing biofilms. Moreover, CHX-like cytotoxicity was observed. [ABSTRACT FROM AUTHOR]

Published
2018
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4. Terpinen-4-ol and carvacrol affect multi-species biofilm composition

Author

Elcio Marcantonio, Denise Madalena Palomari Spolidorio, Tim Verspecht, Martine Pauwels, Wim Teughels, Esteban Rodriguez Herrero, Patricia Milagros Maquera Huacho, Universidade Estadual Paulista (Unesp), and University of Leuven & Dentistry University Hospitals Leuven

Subjects
0301 basic medicine, Peri-implantitis, 030106 microbiology, carvacrol, periodontal disease, Aquatic Science, Applied Microbiology and Biotechnology, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, medicine, Cytotoxic T cell, Humans, Carvacrol, Crystal violet, Water Science and Technology, Terpenes, Chlorhexidine, Terpinen-4-ol, Biofilm, multi-species biofilm, biochemical phenomena, metabolism, and nutrition, 030104 developmental biology, chemistry, Biofilms, Toxicity, Monoterpenes, Cymenes, medicine.drug, peri-implantitis
Abstract

Made available in DSpace on 2019-10-06T17:14:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 The aim of this study was to investigate the cytotoxic activity and inhibitory effect of terpinen-4-ol (T4ol) and carvacrol against single- and multi-species biofilms. The toxicity of each compound was tested on oral keratinocytes and evaluated by XTT assay. Inhibition and eradication of single-species biofilms were analyzed by crystal violet assay and the effect on multi-species biofilm composition was evaluated by qPCR. T4ol and carvacrol did not affect the epithelial cell viability, in contrast to chlorhexidine, which showed a high cytotoxic effect. Inhibition and eradication of single-species biofilms treated with T4ol and carvacrol were observed. The same inhibitory effect was observed for multi-species biofilms, especially on periodontal pathogens. In conclusion, specific concentrations of T4ol and carvacrol without toxicity towards the epithelial cells reduced the numbers of periodontal pathogens in single- and multi-species biofilms. Department of Oral Diagnosis and Surgery School of Dentistry São Paulo State University (Unesp) Department of Oral Health Sciences University of Leuven & Dentistry University Hospitals Leuven Department of Physiology and Pathology School of Dentistry São Paulo State University (Unesp) Department of Oral Diagnosis and Surgery School of Dentistry São Paulo State University (Unesp) Department of Physiology and Pathology School of Dentistry São Paulo State University (Unesp)

Published
2019

5. In vitro antibacterial and cytotoxic activities of carvacrol and terpinen-4-ol against biofilm formation on titanium implant surfaces

Author

Ester Alves Ferreira Bordini, Patricia Milagros Maquera-Huacho, Marília Ferreira Correia, Caroline Coradi Tonon, Renata Serignoli Francisconi, Denise Madalena Palomari Spolidorio, Elcio Marcantonio, and Universidade Estadual Paulista (Unesp)

Subjects
0301 basic medicine, Cell Survival, Microbial Sensitivity Tests, Aquatic Science, Applied Microbiology and Biotechnology, biofilm, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, Minimum inhibitory concentration, Mice, 0302 clinical medicine, antibacterial activity, Carvacrol, Animals, Viability assay, Porphyromonas gingivalis, Cells, Cultured, Water Science and Technology, cytotoxic activity, Titanium, terpinen-4-ol, Minimum bactericidal concentration, biology, Terpenes, Biofilm, 030206 dentistry, biology.organism_classification, Anti-Bacterial Agents, 030104 developmental biology, chemistry, Biofilms, Monoterpenes, Cymenes, Fusobacterium nucleatum, Antibacterial activity, peri-implantitis
Abstract

Made available in DSpace on 2018-12-11T17:38:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01 This study evaluated the antibacterial properties of carvacrol and terpinen-4-ol against Porphyromonas gingivalis and Fusobacterium nucleatum and its cytotoxic effects on fibroblast cells. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were examined. The minimum biofilm inhibition concentration (MBIC) was evaluated by XTT assay. Biofilm decontamination on titanium surfaces was quantified (CFU ml−1), evaluated by confocal laser scanning microscopy (CLSM) and cytotoxic activity by MTT. The MIC and MBC for carvacrol were 0.007% and 0.002% for P. gingivalis and F. nucleatum, and 0.06% for terpinen-4-ol for both microorganisms. The MBIC for carvacrol was 0.03% and 0.06% for P. gingivalis and F. nucleatum, and for terpinen-4-ol was 0.06% and 0.24%. The results indicated anti-biofilm activity using carvacrol (0.26%, 0.06%) and terpinen-4-ol (0.95%, 0.24%) and showed cytotoxic activity similar to chlorohexidine (CHX). However, terpinen-4-ol (0.24%) showed higher cell viability than other treatments. Carvacrol and terpinen-4-ol showed antibacterial activity in respect of reducing biofilms. Moreover, CHX-like cytotoxicity was observed. Department of Oral Diagnosis and Surgery School of Dentistry São Paulo State University (Unesp) Department of Physiology and Pathology School of Dentistry São Paulo State University (Unesp) Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (Unesp) Department of Oral Diagnosis and Surgery School of Dentistry São Paulo State University (Unesp) Department of Physiology and Pathology School of Dentistry São Paulo State University (Unesp) Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (Unesp)

Published
2018

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