1. Viability and membrane lipid composition under a 57mT static magnetic field in Salmonella Hadar
- Author
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Ahmed Landoulsi, Ramla Ben Mouhoub, Alya El May, Mohamed Marouen Sethom, Imen Boujezza, and Moncef Feki
- Subjects
0301 basic medicine ,Cardiolipins ,Membrane lipids ,030106 microbiology ,Biophysics ,Phospholipid ,Adaptation, Biological ,03 medical and health sciences ,chemistry.chemical_compound ,Membrane Lipids ,Salmonella ,Electrochemistry ,Food science ,Physical and Theoretical Chemistry ,chemistry.chemical_classification ,Microbial Viability ,biology ,Chemistry ,Fatty Acids ,Fatty acid ,General Medicine ,biology.organism_classification ,Thin-layer chromatography ,Membrane ,Magnetic Fields ,lipids (amino acids, peptides, and proteins) ,Gas chromatography ,Bacteria - Abstract
The aim of this work is to demonstrate the effects of a static magnetic field (SMF) with an induction 12 equal to 57mT on the viability and membrane lipid composition of Salmonella Hadar. Results showed an increase in the viability of exposed bacteria compared to controls after 9h of exposure. Analysis with gas chromatography of total lipids (TLs) and different fractions of phospholipids: phosphatidylglycerols (PGs), phosphatidylethanolamines (PEs), and cardiolipins (CLs), separated by thin layer chromatography revealed changes in fatty acid levels during exposure. For TLs, the unsaturated fatty acids/saturated fatty acids ratio (UFAs/SFAs) had significantly increased after 9 h of exposure. The variation of this ratio seems to be essentially due to the increase of the proportion of unsaturated fatty acids with 18 carbons, in particular C18:1. The analyses of fatty acid composition carried out on the scale of each fraction of phospholipids showed that CLs contributed significantly to the increase of the proportion of the unsaturated fatty acids between 6 and 9h of exposure thanks to their unsaturated chains with 18 carbons (especially C18:2). CLs appear to be the main phospholipid involved in the adaptation of S. Hadar membranes to the SMF.
- Published
- 2017