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Background: The spectacular decline in pollinators and their prominent role in pollination of natural and cultivated plants has stimulated research on pollinating insects. Over the last ten years, much ecological research has been carried out on bees, often generating a large volume of specimens and increasing the importance of entomological collections. Here, we present the bee collection of the IMBE laboratory (Marseille, France) after ten years of study of plant-pollinator networks. New information: We provide distribution data on 2181 specimens belonging to 246 species of bees, mainly from the Mediterranean Region of France. One of the recorded species, Lasioglossum soror, is classified as "endangered" at the European level, while 68 of the recorded species are currently Data Deficient according to the 2014 Red List of European bees. This dataset contributes to the broader effort to enhance the knowledge of French bee diversity. It aligns with the objectives of the French Pollinator Plan and supports the development of a national Red List. In this context, information about the distribution of wild bees from the Mediterranean Region, which harbours the highest species diversity in mainland France, are of particular importance. [ABSTRACT FROM AUTHOR]