1. Toward an in vitro test for the diagnosis of allergy to penicillins. Synthesis, characterization, and use of beta-lactam and beta-lactam metabolite poly-L-lysines which recognize human IgE antibodies
- Author
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Paola Gramatica, Roberto Todeschini, Marco Orlandi, Bruno Rindone, Viviana Consonni, Ezio Bolzacchini, Simone Meinardi, Cristobalina Mayorga, Miguel Blanca, Bolzacchini, E, Consonni, V, Gramatica, P, Meinardi, S, Orlandi, M, Rindone, B, and Todeschini, R
- Subjects
Allergy ,Magnetic Resonance Spectroscopy ,Stereochemistry ,Metabolite ,Biomedical Engineering ,Pharmaceutical Science ,Allergy to Penicillin ,Bioengineering ,Penicillins ,Immunoglobulin E ,beta-Lactams ,Drug Hypersensitivity ,chemistry.chemical_compound ,Residue (chemistry) ,Radioallergosorbent Test ,polycyclic compounds ,medicine ,Humans ,Polylysine ,Chromatography, High Pressure Liquid ,Pharmacology ,Poly-l-lysine ,biology ,medicine.diagnostic_test ,Radioallergosorbent test ,Organic Chemistry ,Nuclear magnetic resonance spectroscopy ,medicine.disease ,Penicillin ,chemistry ,Biochemistry ,CHIM/12 - CHIMICA DELL'AMBIENTE E DEI BENI CULTURALI ,Desensitization, Immunologic ,β-Lactam and β-Lactam Metabolite ,biology.protein ,Human IgE Antibodies ,Antibody ,Biotechnology ,medicine.drug - Abstract
Conjugates of poly-L-lysine (PLL) containing a penicillin or a penicilloyl residue were prepared and characterized by H-1 NMR and by size-exclusion (SE) HPLC. These conjugates were used in a radio allergo sorbent tests (RAST) test for the determination of allergy toward beta-lactams. The chemiometric evaluation of the data indicates that allergy to amoxycillin is different from allergy to the ether beta-lactams tested. Furthermore, careful chemical characterization of the conjugates appears to be crucial to obtain meaningful information from the RAST data.
- Published
- 1999