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8-Oxyguanine (8ohG) is a major oxidation product of guanine and a biomarker of oxidative stress in mammal. We have attempted to estimate the level of 8ohG residues in plasmid DNA (pGW2123 and pBR322) grown in various bacterial strains (fpg, mutY, or mutT, plus mutY fpgand mutT mutYdouble mutants) differing in the system protecting cells against the mutagenic effects of 8ohG in DNA. The method was based on digestion of plasmid DNA with Fpg, and agarose gel electrophoresis. Fpg converts pDNA from covalently closed circular to the open circular (ccc→oc) form of pDNA when there is at least one 8ohG, or apurinic site, per cccpDNA molecule. It was found that: i) the content of 8ohG in pDNA grown in any of the tested bacteria is below one 8ohG per 104base pairs; ii) a substantial part of pGW2123 is isolated from the bacteria in the ocform; iii) the ratio of oc/cccin pGW2123 depends on the bacterial host and is the lowest when the plasmid was harvested from mutY−deficient cells; iv) pBR322, unlike pGW2123, is isolated predominantly in the cccform; and v) of the pBR322 grown in the tested bacteria apparently the most resistant to Fpg digestion was pBR322 grown in the mutYstrain. It is proposed that this reflects the compact structure of pDNAs when they are grown in bacteria deficient in mutYgene product.