1. Long chain fatty acyl-CoA synthetase 5 expression is induced by insulin and glucose: involvement of sterol regulatory element-binding protein-1c
- Author
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Isabelle Hainault, Pascal Ferré, Dominique Bécard, D Allanic, Fabienne Foufelle, Younes Achouri, and Bronwyn D. Hegarty
- Subjects
medicine.medical_specialty ,Anabolism ,medicine.medical_treatment ,Biochemistry ,Polymerase Chain Reaction ,Diabetes Mellitus, Experimental ,Mitochondrial Proteins ,Eating ,Internal medicine ,Diabetes mellitus ,Coenzyme A Ligases ,medicine ,Animals ,Insulin ,RNA, Messenger ,Rats, Wistar ,biology ,Fatty Acids ,General Medicine ,Fasting ,Carbohydrate ,medicine.disease ,Sterol regulatory element-binding protein ,Rats ,Insulin receptor ,Endocrinology ,medicine.anatomical_structure ,Glucose ,Liver ,Hepatocyte ,Enzyme Induction ,Lipogenesis ,Models, Animal ,biology.protein ,lipids (amino acids, peptides, and proteins) ,Female ,Sterol Regulatory Element Binding Protein 1 - Abstract
In a screen for sterol regulatory element-binding protein (SREBP)-1c target genes in the liver, we identified long chain fatty acyl-CoA synthetase 5 (ACS-5). Hepatic ACS-5 mRNA is poorly expressed during fasting and diabetes and strongly induced by carbohydrate refeeding and insulin treatment. In cultured hepatocytes, insulin and a high glucose concentration induce ACS-5 mRNA. Adenoviral overexpression of a nuclear form of SREBP-1c in liver of diabetic mice or in cultured hepatocytes mimics the effect of insulin to induce ACS-5. By contrast, a dominant negative form of SREBP-1c abolishes the effect of insulin on ACS-5 expression. The dietary and SREBP-1c-mediated insulin regulation of ACS-5 expression indicate that ACS-5 is involved in the anabolic fate of fatty acids.
- Published
- 2005