1. α-Enolase binds to RNA
- Author
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Hernández-Pérez, Liliana, Depardón, Francisco, Fernández- Ramírez, Fernando, Sánchez-Trujillo, Alejandra, Bermúdez-Crúz, Rosa María, Dangott, Lawrence, and Montañez, Cecilia
- Subjects
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ENOLASE , *RNA , *MASS spectrometry , *CARRIER proteins , *RADIOLABELING , *RNA probes , *GLYCOLYSIS - Abstract
Abstract: To detect proteins binding to CUG triplet repeats, we performed magnetic bead affinity assays and North-Western analysis using a (CUG)10 ssRNA probe and either nuclear or total extracts from rat L6 myoblasts. We report the isolation and identification by mass spectrometry and immunodetection of α-enolase, as a novel (CUG)n triplet repeat binding protein. To confirm our findings, rat recombinant α-enolase was cloned, expressed and purified; the RNA binding activity was verified by electrophoretic mobility shift assays using radiolabeled RNA probes. Enolase may play other roles in addition to its well described function in glycolysis. [Copyright &y& Elsevier]
- Published
- 2011
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