1. Galectin-3 S-glutathionylation regulates its effect on adipocyte insulin signaling
- Author
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Mohita Maurya, Anant Jaiswal, Sanchita Gupta, Wahid Ali, Anil Nilkanth Gaikwad, Madhu Dikshit, and Manoj Kumar Barthwal
- Subjects
Mice ,Galectin 3 ,Adipocytes ,Animals ,Insulin ,Cell Biology ,Hydrogen Peroxide ,Molecular Biology ,Signal Transduction - Abstract
Protein-S-glutathionylation promotes redox signaling in physiological and oxidative distress conditions. Galectin-3 (Gal-3) promotes insulin resistance by down-regulating adipocyte insulin signaling, however, its S-glutathionylation and significance is not known. In this context, we report reversible S-glutathionylation of Gal-3. Site-directed mutagenesis established Gal-3 Cys187 as the putative S-glutathionylation site. Glutathionylated Gal-3 prevents Gal-3(WT)-Insulin Receptor interaction and facilitates insulin-induced murine adipocyte p-IRS1(tyr895) and p-AKT(ser473) signaling and glucose uptake in a Gal-3 Cys187 glutathionylation dependent manner in murine adipocytes, as assessed by Western blotting and 2-NBDG uptake assay respectively. Pre-glutathionylated Gal-3 at Cys187 resisted irreversible oxidation by H
- Published
- 2021