1. Genomic DNA sequence of a rice gene coding for a pullulanase-type of starch debranching enzyme
- Author
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Shin Young Park, Perigio B. Francisco, Yi Zhang, Naoki Ogata, Yasunori Nakamura, and Hiroaki Yamanouchi
- Subjects
Glycoside Hydrolases ,Molecular Sequence Data ,Restriction Mapping ,Biophysics ,Sequence alignment ,Biology ,Genes, Plant ,Biochemistry ,Exon ,Structural Biology ,RNA, Messenger ,Cloning, Molecular ,Molecular Biology ,Gene ,Conserved Sequence ,Genetics ,Base Sequence ,Pullulanase ,Accession number (library science) ,Intron ,food and beverages ,Hordeum ,Oryza ,Exons ,Sequence Analysis, DNA ,Introns ,genomic DNA ,GenBank ,Sequence Alignment - Abstract
A genomic DNA containing a rice (Oryza sativa L., cv. Norin-8) gene coding for a pullulanase-type starch debranching enzyme (EC 3.2.1. 41) was sequenced (EMBL/GenBank/DDBJ accession number AB012915). Along the 15, 248 bp DNA, the pullulanase gene is split into 26 exons. The four pullulanase consensus regions are positioned in the middle portion of the sequence and are separated by long introns and 1-3 exons. Comparison of the rice cv. Norin-8 pullulanase genomic structure with that of barley pullulanase (limit dextrinase) (F. Lok et al., EMBL/GenBank/DDBJ accession number AF022725) indicates that most of the pullulanase exons are highly conserved. Alignment of the nucleotide bases of rice exon 8 with those of barley exon 8-intron 8-exon 9 fragment suggests that the 85 bp internal sequence of rice exon 8 was originally an intron, a possibility further indicated by the absence in barley and spinach (A. Renz et al., EMBL/GenBank/DDBJ accession number X83969) pullulanases of amino acid residues encoded by the 85 bp fragment.
- Published
- 1998
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