1. The role of calcium in fly salivary gland secretion analyzed with the ionophore A-23187
- Author
-
William T. Prince, Howard Rasmussen, and Michael J. Berridge
- Subjects
Serotonin ,medicine.medical_specialty ,Time Factors ,Carboxylic Acids ,Biophysics ,Ionophore ,chemistry.chemical_element ,In Vitro Techniques ,Biology ,Calcium ,Tritium ,Models, Biological ,Biochemistry ,Epithelium ,Salivary Glands ,Membrane Potentials ,Houseflies ,Internal medicine ,Cyclic AMP ,medicine ,Animals ,Secretion ,Molecular Biology ,Salivary gland ,Adenine ,Calcium Radioisotopes ,Salivary gland secretion ,Adenosine Monophosphate ,Anti-Bacterial Agents ,Kinetics ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Second messenger system ,Efflux ,Calcium influx - Abstract
The ionophore A-23187 at a concentration of 10 −6 M stimulates fluid secretion, increases calcium influx and efflux, and alters the trensepithelial potential in the isolated fly salivary gland but causes a fall in cyclic AMP concentration. In this regard, it mimics all the effects of 5-hydroxytryptamine of this tissue, except that 5-hydroxytryptamine causes a rise in cyclic AMP concentration. These data in conjunction with past evidence indicate that calcium, as well as cyclic AMP, is a second messenger in the action of 5-hydroxytryptamine upon fly salivary gland secretion.
- Published
- 1973