1. Investigation of the thermodynamic drivers of the interaction between the high mobility group box domain of Sox2 and bacterial lipopolysaccharide
- Author
-
Shweta Shetty, Ernest D. Pianim, Patrick H. Hewitt, Christopher S. Malarkey, Nicholas A. DiCesare, Casey Gray, Jan V. Bernal, Trung T. Leong, and Kuriko Sakai
- Subjects
Lipopolysaccharides ,0301 basic medicine ,Lipopolysaccharide ,Biophysics ,Inflammation ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Immune system ,SOX2 ,medicine ,Humans ,Receptor ,Helicobacter pylori ,biology ,Chemistry ,SOXB1 Transcription Factors ,fungi ,Cancer ,Cell Biology ,biology.organism_classification ,medicine.disease ,Molecular Docking Simulation ,030104 developmental biology ,High-mobility group ,HMG-Box Domains ,030220 oncology & carcinogenesis ,embryonic structures ,Cancer research ,medicine.symptom ,Protein Binding - Abstract
Gastric cancer is associated with high mortality and is preceded by an infection with Helicobacter pylori (H. pylori). H. pylori stimulates inflammation which involves the activation of Toll-like receptor 4 by lipopolysaccharide molecules from the H. pylori. This leads to chronic inflammation that can eventually lead to gastric cancer. Sox2 is a member of the high mobility group (HMG) box family of proteins, and recent studies have shown that HMG box proteins can modulate immune response by altering signaling to Toll-like receptors. Sox2 is overexpressed in most types of cancer with the exception of gastric cancer where expression of Sox2 is decreased. Here, we demonstrate that Sox2 can bind LPS and we investigated the thermodynamic drivers of the Sox2/LPS interaction.
- Published
- 2020