1. RNA: RNA interactions in the large subunit ribosomal RNA of Euglena gracilis.
- Author
-
Smallman DS, Schnare MN, and Gray MW
- Subjects
- Animals, Base Sequence, Cytoplasm metabolism, Electrophoresis, Polyacrylamide Gel, Euglena gracilis genetics, Models, Molecular, Molecular Sequence Data, Molecular Structure, Nucleic Acid Conformation, RNA Processing, Post-Transcriptional, RNA, Protozoan chemistry, RNA, Protozoan genetics, RNA, Ribosomal chemistry, RNA, Ribosomal genetics, RNA, Ribosomal, 28S chemistry, RNA, Ribosomal, 28S genetics, RNA, Ribosomal, 28S metabolism, RNA, Ribosomal, 5.8S chemistry, RNA, Ribosomal, 5.8S genetics, RNA, Ribosomal, 5.8S metabolism, Ribosomes, Euglena gracilis metabolism, RNA, Protozoan metabolism, RNA, Ribosomal metabolism
- Abstract
In Euglena gracilis, the cytoplasmic large subunit (LSU) rRNA is composed of 14 discrete small RNA species that must somehow interact in the functional ribosome. We have isolated native complexes of Euglena rRNA and show here that the largest of these complexes contains eight of the 14 LSU rRNA species. Several of these small rRNA species are able to associate in vitro to reform an isolated domain of LSU rRNA structure.
- Published
- 1996
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