1. The origin of Listeria monocytogenes 4b isolates is signified by subproteomic profiling
- Author
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Bruno Meunier, Mickaël Desvaux, Jean-Louis Berdagué, Christophe Chambon, Michel Hébraud, Emilie Dumas, Microbiologie Equipe Qualité et Sécurité des Aliments (INRA), Institut National de la Recherche Agronomique (INRA), Unité de Recherches sur les Herbivores (URH), Qualité des Produits Animaux Equipe Typicité Aromatique et Authentification (INRA), Plateforme Proteomique (INRA), Herbivores Equipe Croissance et Métabolisme du Muscle (INRA), SECurité des ALIments et Microbiologie, Institut National de la Recherche Agronomique (INRA)-École nationale d'ingénieurs des techniques des industries agricoles et alimentaires (ENITIAA)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Unité de Nutrition Humaine (UNH), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), and Unité de Microbiologie (MIC)
- Subjects
Serotype ,Proteomics ,Proteome ,Biophysics ,Protein Array Analysis ,Gene Expression ,Extracellular proteome ,Biology ,medicine.disease_cause ,Biochemistry ,Peptide Mapping ,Analytical Chemistry ,Microbiology ,03 medical and health sciences ,Listeria monocytogenes ,Bacterial Proteins ,medicine ,Food microbiology ,Humans ,Cytoplasmic proteome ,Electrophoresis, Gel, Two-Dimensional ,Listeriosis ,Serotyping ,Peptide-mass fingerprint ,Molecular Biology ,Gene ,030304 developmental biology ,Gel electrophoresis ,0303 health sciences ,030306 microbiology ,MALDI-TOF mass spectrometry ,Biodiversity ,Serovar 4b ,Genes, Bacterial ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Food Microbiology ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology - Abstract
International audience; Among the 13 known serovars of Listeria monocytogenes, strains exhibiting the serovar 4b are the most prevalently involved in epidemics of human listeriosis. The molecular reasons for the major involvement of serovar 4b strains in all major foodborne outbreaks in contrast to the lower prevalence of this serovar among food isolates remain indefinite. In order to provide further insight in the protein expression of L. monocytogenes 4b strains, the cytoplasmic and extracellular proteomes of L. monocytogenes 4b strains from different origins, i.e. environmental, clinical and asymptomatical carriage, were investigated by two-dimensional gel electrophoresis. Statistical hierarchical clustering analysis on subproteomic profiles clearly discriminated the strains according to their origin. Protein spots differentiating the subproteome patterns were identified using MALDI-TOF MS through their peptide mass fingerprint.
- Published
- 2009
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