1. Bay K8644 like activity of an antibody against a 60 kDa tubular membrane protein
- Author
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Pulak Lahiri, Diptendu Chatterjee, Munmun Chakraborty, and Amitabha Chatterjee
- Subjects
Nifedipine ,Biophysics ,Receptors, Nicotinic ,Biochemistry ,Antibodies ,Cell membrane ,chemistry.chemical_compound ,Mice ,Cell surface receptor ,Heart Rate ,medicine ,Animals ,Receptor ,Cells, Cultured ,Antiserum ,Mice, Inbred BALB C ,biology ,Ryanodine receptor ,Calcium channel ,Muscles ,Membrane Proteins ,Cell Biology ,3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester ,Bay K8644 ,Rats ,Molecular Weight ,medicine.anatomical_structure ,chemistry ,biology.protein ,Calcium Channels ,Antibody - Abstract
Partial purification of the dihydropyridine receptor from rat skeletal muscle demonstrated mainly a 60 kDa band in SDS-polyacrylamide gel. An antibody raised against that protein behaved as a calcium channel agonist viz. Bay K8644. The affinity purified antibody, when added to cultured heart cells, increased the beat rate 40–80% depending on the titer of the antiserum. The antibody also woke up the beats of the cells previously blocked with the channel antagonist, nifedipine. Immunoblot analysis indicated that the receptor of this antibody in heart cell membrane is also a 60 kDa protein.
- Published
- 1989