1. Type 1 Metallothionein (ZjMT) Is Responsible for Heavy Metal Tolerance in Ziziphus jujuba.
- Author
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Li LS, Meng YP, Cao QF, Yang YZ, Wang F, Jia HS, Wu SB, and Liu XG
- Subjects
- Amino Acid Sequence, Cadmium metabolism, Cadmium toxicity, Cloning, Molecular, DNA, Complementary genetics, DNA, Complementary metabolism, Electrophoresis, Polyacrylamide Gel, Escherichia coli drug effects, Escherichia coli metabolism, Metallothionein chemistry, Metallothionein genetics, Metals, Heavy toxicity, Molecular Sequence Data, Plasmids genetics, Plasmids metabolism, Real-Time Polymerase Chain Reaction, Recombinant Fusion Proteins biosynthesis, Recombinant Fusion Proteins chemistry, Recombinant Fusion Proteins isolation & purification, Sequence Alignment, Ziziphus drug effects, Metallothionein metabolism, Metals, Heavy metabolism, Ziziphus metabolism
- Abstract
Metallothioneins (MTs) are a family of low molecular weight, cysteine-rich, metal-binding proteins that are able to make cells to uptake heavy metals from the environment. Molecular and functional characterization of this gene family improves understanding of the mechanisms underlying heavy metal tolerance in higher organisms. In this study, a cDNA clone, encoding 74-a.a. metallothionein type 1 protein (ZjMT), was isolated from the cDNA library of Ziziphus jujuba. At the N- and C-terminals of the deduced amino acid sequence of ZjMT, six cysteine residues were arranged in a CXCXXXCXCXXXCXC and CXCXXXCXCXXCXC structure, respectively, indicating that ZjMT is a type 1 MT. Quantitative PCR analysis of plants subjected to cadmium stress showed enhanced expression of ZjMT gene in Z. jujuba within 24 h upon Cd exposure. Escherichia coli cells expressing ZjMT exhibited enhanced metal tolerance and higher accumulation of metal ions compared with control cells. The results indicate that ZjMT contributes to the detoxification of metal ions and provides marked tolerance against metal stresses. Therefore, ZjMT may be a potential candidate for tolerance enhancement in vulnerable plants to heavy metal stress and E. coli cells containing the ZjMT gene may be applied to adsorb heavy metals in polluted wastewater.
- Published
- 2016
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