1. Identification of proteins overexpressed in papillary thyroid tumors.
- Author
-
Sipina LV, Bukurova YA, Nikitina IG, Krasnov GS, Sergeev SA, Lisitsyn NA, Karpov VL, and Beresten SF
- Subjects
- Biomarkers, Tumor metabolism, Carcinoma, Carcinoma, Papillary, Electrophoresis, Gel, Two-Dimensional, Humans, Mass Spectrometry, Thyroid Cancer, Papillary, Proteome metabolism, Proteomics methods, Thyroid Neoplasms metabolism
- Abstract
A modified method of proteome comparative analysis based on preliminary removal of cell structural proteins by extraction using salt buffer and subsequent separation of extracts by two-dimensional gel electrophoresis was developed. Identification of differentially expressed proteins by mass spectrometry has revealed three proteins with noticeably increased level of synthesis in most samples of papillary thyroid tumors compared to normal tissues. An increase in ubiquitin content was found for the first time. Oncomarker search efficiencies by two-dimensional gel electrophoresis and bioinformatic search were compared.
- Published
- 2010
- Full Text
- View/download PDF