1. A novel protein from Eupolyphaga sinensis inhibits adhesion, migration, and invasion of human lung cancer A549 cells
- Author
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Ai-guo Ji, Jin Zhao, Jia Liu, Jianteng Wei, Fengxia Wang, Ning Wu, and Xiukun Lin
- Subjects
Insecta ,Lung Neoplasms ,Time Factors ,Antineoplastic Agents ,Apoptosis ,Cockroaches ,Biochemistry ,Eupolyphaga sinensis ,Sepharose ,Inhibitory Concentration 50 ,Cell Movement ,Cell Line, Tumor ,Cell Adhesion ,Animals ,Humans ,Neoplasm Invasiveness ,Cell adhesion ,Molecular Biology ,Ammonium sulfate precipitation ,A549 cell ,biology ,Chemistry ,Integrin beta1 ,Cell migration ,Cell Biology ,Chromatography, Ion Exchange ,biology.organism_classification ,Fibronectin ,Chromatography, Gel ,biology.protein ,Insect Proteins ,Drug Screening Assays, Antitumor - Abstract
Eupolyphaga sinensis Walker is an important insect used in Chinese traditional medicine. In this study, we purified a 72-kDa anticancer protein, designated as EPS72, from this species using ammonium sulfate precipitation, ultrafiltration, CM Sepharose Fast Flow cation exchange, Q Sepharose High Performance (HP) anion exchange, Butyl Sepharose HP hydrophobic chromatography, and Superdex 75 gel filtration chromatographic techniques. EPS72 exhibited a potent anticancer activity against the human lung cancer A549 cell line (IC50, 18.76 μg/mL). Further study showed that EPS72 could induce A549 cell detachment and apoptosis, inhibit cell adhesion to fibronectin and collagen IV, and restrain cell migration and invasion. Moreover, EPS72 significantly decreased the expression of β1-integrin. This study suggests that EPS72 could potentially be developed as a novel anticancer therapeutic agent due to its possible antimetastatic activity.
- Published
- 2013
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