1. IGF2BP1-Mediated Regulation of CCN1 Expression by Specific Binding to G-Quadruplex Structure in Its 3'UTR.
- Author
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Rana P, Ujjainiya R, Bharti V, Maiti S, and Ekka MK
- Subjects
- Humans, RNA, Messenger genetics, RNA, Messenger metabolism, RNA Stability, Gene Expression Regulation, Protein Binding, G-Quadruplexes, RNA-Binding Proteins metabolism, RNA-Binding Proteins genetics, RNA-Binding Proteins chemistry, 3' Untranslated Regions genetics, Cysteine-Rich Protein 61 metabolism, Cysteine-Rich Protein 61 genetics, Cysteine-Rich Protein 61 chemistry
- Abstract
The intricate regulation of gene expression is fundamental to the biological complexity of higher organisms, and is primarily governed by transcriptional and post-transcriptional mechanisms. The 3'-untranslated region (3'UTR) of mRNA is rich in cis-regulatory elements like G-quadruplexes (G4s), and plays a crucial role in post-transcriptional regulation. G4s have emerged as significant gene regulators, impacting mRNA stability, translation, and localization. In this study, we investigate the role of a robust parallel G4 structure situated within the 3'UTR of CCN1 mRNA in post-transcriptional regulation. This G4 structure is proximal to the stop codon of human CCN1, and evolutionarily conserved. We elucidated its interaction with the insulin-like growth factor 2 binding protein 1 (IGF2BP1), a noncanonical RNA N6-methyladenosine (m6A) modification reader, revealing a novel interplay between RNA modifications and G-quadruplex structures. Knockdown experiments and mutagenesis studies demonstrate that IGF2BP1 binds specifically to the G4 structure, modulating CCN1 mRNA stability. Additionally, we unveil the role of IGF2BP1's RNA recognition motifs in G4 recognition, highlighting this enthalpically driven interaction. Our findings offer fresh perspectives on the complex mechanisms of post-transcriptional gene regulation mediated by G4 RNA secondary structures.
- Published
- 2024
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