Search

Your search keyword '"Savagnac A"' showing total 2 results
Start Over Author "Savagnac A" Journal biochemistry
2 results on '"Savagnac A"'

1. Structures of nodulation factors from the nitrogen-fixing soybean symbiont Rhizobium fredii USDA257

Author

Bec-Ferte, Marie-Pierre, Krishnan, Hari B., Prome, Danielle, Savagnac, Arlette, Pueppke, Steven G., and Prome, Jean-Claude

Subjects
Rhizobium -- Research, Nitrogen -- Fixation, Biological sciences, Chemistry
Abstract

Isoflavone genistein stimulates Rhizobium fredii USDA257 to synthesize a family of oligochitin nodulation factors, which can be extracted using methanol. Thin-layer chromatographic and mass spectrometric characterization of these Nod factors reveal these factors as substituted, beta1,4-linked tri-, tetra- and pentamers of N-acetylglucosamine. The Nod factors have an N-vaccenoyl group on the nonreducing residue. The reducing residue has an alpha-linked 2-O-methylfucose on carbon 6. Examination of the biological activities of these Nod factors reveals that different Nod factors exhibit different influence on the root-hair deformation on three legume species. The concentration of the Nod factor influences the biological activity.

Published
1994

2. Structures of Nodulation Factors from the Nitrogen-Fixing Soybean Symbiont Rhizobium fredii USDA257

Author

Steven G. Pueppke, Hari B. Krishnan, Arlette Savagnac, Marie Pierre Bec-Ferté, Danielle Promé, and Jean-Claude Adrien Paul Prome

Subjects
Molecular Sequence Data, Oligosaccharides, Genistein, Plant Roots, Biochemistry, Mass Spectrometry, Acetylglucosamine, chemistry.chemical_compound, Tetramer, Nitrogen Fixation, Extracellular, Symbiosis, Chromatography, High Pressure Liquid, Legume, Residue (complex analysis), Plants, Medicinal, biology, food and beverages, Fabaceae, biology.organism_classification, Carbohydrate Sequence, chemistry, Glycine, Nitrogen fixation, Rhizobium, Biological Assay
Abstract

We have isolated and characterized the extracellular Nod factors of Rhizobium fredii USDA257, a nitrogen-fixing symbiont of soybean [Glycine max (L.) Merr.] and several other legume species. These signals are produced upon exposure to the isoflavone genistein and consist of a series of substituted, beta 1,4-linked tri-, tetra-, and pentamers of N-acetylglucosamine. N-Vaccenic acid replaces acetate on the nonreducing residue, and the reducing residue contains alpha-linked 2-O-methylfucose on carbon 6. Small amounts of a fucose-containing tetramer also were present. The Nod factors elicit root-hair deformations on soybean and two other plants at concentrations ranging from 10(-6) to 10(-12) M.

Published
1994
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results