1. MbtH-like proteins as integral components of bacterial nonribosomal peptide synthetases.
- Author
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Felnagle EA, Barkei JJ, Park H, Podevels AM, McMahon MD, Drott DW, and Thomas MG
- Subjects
- Bacteria genetics, Bacterial Proteins genetics, Peptide Synthases genetics, Bacteria enzymology, Bacterial Proteins metabolism, Capreomycin biosynthesis, Multigene Family physiology, Peptide Synthases metabolism, Viomycin biosynthesis
- Abstract
The biosynthesis of many natural products of clinical interest involves large, multidomain enzymes called nonribosomal peptide synthetases (NRPSs). In bacteria, many of the gene clusters coding for NRPSs also code for a member of the MbtH-like protein superfamily, which are small proteins of unknown function. Using MbtH-like proteins from three separate NRPS systems, we show that these proteins copurify with the NRPSs and influence amino acid activation. As a consequence, MbtH-like proteins are integral components of NRPSs.
- Published
- 2010
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