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Start Over Author "Brent Thomas" Journal biochemistry
7 results on '"Brent Thomas"'

1. Characterization of cDNA encoding mouse DNA repair protein O(super 6)-methylguanine-DNA methyltransferase and high-level expression of the wild-type and mutant proteins in Escherichia coli

Author

Shiota, Susumu, Wronski, Matthew A. von, Tano, Keizo, Bigner, Darell D., Brent, Thomas P., and Mitra, Sankar

Subjects
Methyltransferases -- Research, Escherichia coli -- Research, DNA -- Research, Biological sciences, Chemistry
Abstract

The O(super 6)-methylguanine-DNA methyltransferase (MGMT) gene cloned from a lambdagt11 mouse cDNA library was isolated and characterized. MGMT repairs the mutagenic O(super 6)-alkylguanine lesion in DNA. The 22kDa protein contains 211 amino acids and 70% of its deduced nucleotide sequence was found homologous to human MGMT. A T7 promoter-based vector for high-level expression was constructed by site-directed mutagenesis for the large-scale production of wild-type and mutant mouse MGMTs.

Published
1992

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