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Start Over Author "Wu, Jianhua" Topic enzymes Journal biochemistry
1 results on '"Wu, Jianhua"'

1. Cysteine 148 in the lactose permease of Escherichia coli is a component of a substrate binding site.

Author

Wu, Jianhua and Kaback, H. Ronald

Subjects
*ENZYMES, *AMINO acids, *BACTERIAL genetics, *ESCHERICHIA coli, *FLUORESCENCE spectroscopy, *REACTIVITY (Chemistry), *STRUCTURE-activity relationships
Abstract

Describes the use of site-directed fluorescence labeling to provide more direct evidence for the contention that cysteine (Cys) 148 is located in a binding site in the lactose (lac) permease of Escherichia coli. Plasmid construction; Purification of biotinated lac permease with single Cys residues; Protein determinations; Fluorescence quenching studies.

Published
1994
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